Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance
available in PMC 2016 March 04
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| מחברים אחרים: | |
| פורמט: | Article |
| שפה: | en_US |
| יצא לאור: |
Elsevier B.V.
2017
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| גישה מקוונת: | http://hdl.handle.net/1721.1/107738 https://orcid.org/0000-0002-0419-3351 |
| _version_ | 1826199383319773184 |
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| author | Madisen, Linda Garner, Aleena R. Shimaoka, Daisuke Li, Lu van der Bourg, Alexander Niino, Yusuke Egolf, Ladan Monetti, Claudio Gu, Hong Mills, Maya Cheng, Adrian Tasic, Bosiljka Nguyen, Thuc Nghi Sunkin, Susan M. Benucci, Andrea Nagy, Andras Miyawaki, Atsushi Helmchen, Fritjof Empson, Ruth M. Knöpfel, Thomas Reid, R. Clay Carandini, Matteo Zeng, Hongkui Chuong, Amy S Klapoetke, Nathan Cao Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Madisen, Linda Garner, Aleena R. Shimaoka, Daisuke Li, Lu van der Bourg, Alexander Niino, Yusuke Egolf, Ladan Monetti, Claudio Gu, Hong Mills, Maya Cheng, Adrian Tasic, Bosiljka Nguyen, Thuc Nghi Sunkin, Susan M. Benucci, Andrea Nagy, Andras Miyawaki, Atsushi Helmchen, Fritjof Empson, Ruth M. Knöpfel, Thomas Reid, R. Clay Carandini, Matteo Zeng, Hongkui Chuong, Amy S Klapoetke, Nathan Cao Boyden, Edward |
| author_sort | Madisen, Linda |
| collection | MIT |
| description | available in PMC 2016 March 04 |
| first_indexed | 2024-09-23T11:19:22Z |
| format | Article |
| id | mit-1721.1/107738 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:19:22Z |
| publishDate | 2017 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/1077382022-10-01T02:49:02Z Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance Madisen, Linda Garner, Aleena R. Shimaoka, Daisuke Li, Lu van der Bourg, Alexander Niino, Yusuke Egolf, Ladan Monetti, Claudio Gu, Hong Mills, Maya Cheng, Adrian Tasic, Bosiljka Nguyen, Thuc Nghi Sunkin, Susan M. Benucci, Andrea Nagy, Andras Miyawaki, Atsushi Helmchen, Fritjof Empson, Ruth M. Knöpfel, Thomas Reid, R. Clay Carandini, Matteo Zeng, Hongkui Chuong, Amy S Klapoetke, Nathan Cao Boyden, Edward Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Chuong, Amy S Klapoetke, Nathan Cao Boyden, Edward available in PMC 2016 March 04 An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity. National Institutes of Health (U.S.) (NIH grant DA028298) Wellcome Trust (London, England) (Grant) National Institutes of Health (U.S.) (NIH grant MH085500) 2017-03-27T20:35:38Z 2017-03-27T20:35:38Z 2015-03 2015-01 Article http://purl.org/eprint/type/JournalArticle 08966273 http://hdl.handle.net/1721.1/107738 Madisen, Linda, Aleena R. Garner, Daisuke Shimaoka, Amy S. Chuong, Nathan C. Klapoetke, Lu Li, Alexander van der Bourg, et al. "Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance." Neuron vol.85, issue 5 (4 March 2015), pp. 942–958. https://orcid.org/0000-0002-0419-3351 en_US http://dx.doi.org/10.1016/j.neuron.2015.02.022 Neuron Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier B.V. PMC |
| spellingShingle | Madisen, Linda Garner, Aleena R. Shimaoka, Daisuke Li, Lu van der Bourg, Alexander Niino, Yusuke Egolf, Ladan Monetti, Claudio Gu, Hong Mills, Maya Cheng, Adrian Tasic, Bosiljka Nguyen, Thuc Nghi Sunkin, Susan M. Benucci, Andrea Nagy, Andras Miyawaki, Atsushi Helmchen, Fritjof Empson, Ruth M. Knöpfel, Thomas Reid, R. Clay Carandini, Matteo Zeng, Hongkui Chuong, Amy S Klapoetke, Nathan Cao Boyden, Edward Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance |
| title | Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance |
| title_full | Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance |
| title_fullStr | Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance |
| title_full_unstemmed | Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance |
| title_short | Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance |
| title_sort | transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance |
| url | http://hdl.handle.net/1721.1/107738 https://orcid.org/0000-0002-0419-3351 |
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