Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons
While gustatory sensing of the five primary flavors (sweet, salty, sour, bitter, and savory) has been extensively studied, pathways that detect non-canonical taste stimuli remain relatively unexplored. In particular, while reactive oxygen species cause generalized damage to biological systems, no gu...
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| Format: | Article |
| Language: | en_US |
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Elsevier/Cell Press
2017
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| Online Access: | http://hdl.handle.net/1721.1/107846 https://orcid.org/0000-0002-1693-4524 https://orcid.org/0000-0002-9964-9613 |
| _version_ | 1811097129579446272 |
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| author | Bhatla, Nikhil Horvitz, Howard Robert |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Bhatla, Nikhil Horvitz, Howard Robert |
| author_sort | Bhatla, Nikhil |
| collection | MIT |
| description | While gustatory sensing of the five primary flavors (sweet, salty, sour, bitter, and savory) has been extensively studied, pathways that detect non-canonical taste stimuli remain relatively unexplored. In particular, while reactive oxygen species cause generalized damage to biological systems, no gustatory mechanism to prevent ingestion of such material has been identified in any organism. We observed that light inhibits C. elegans feeding and used light as a tool to uncover molecular and neural mechanisms for gustation. Light can generate hydrogen peroxide, and we discovered that hydrogen peroxide similarly inhibits feeding. The gustatory receptor family members LITE-1 and GUR-3 are required for the inhibition of feeding by light and hydrogen peroxide. The I2 pharyngeal neurons increase calcium in response to light and hydrogen peroxide, and these
responses require GUR-3 and a conserved antioxidant enzyme peroxiredoxin PRDX-2. Our results demonstrate a gustatory mechanism that mediates the detection and blocks ingestion of a noncanonical
taste stimulus, hydrogen peroxide. |
| first_indexed | 2024-09-23T16:54:48Z |
| format | Article |
| id | mit-1721.1/107846 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:54:48Z |
| publishDate | 2017 |
| publisher | Elsevier/Cell Press |
| record_format | dspace |
| spelling | mit-1721.1/1078462022-09-29T22:23:54Z Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons Bhatla, Nikhil Horvitz, Howard Robert Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Bhatla, Nikhil Horvitz, Howard Robert While gustatory sensing of the five primary flavors (sweet, salty, sour, bitter, and savory) has been extensively studied, pathways that detect non-canonical taste stimuli remain relatively unexplored. In particular, while reactive oxygen species cause generalized damage to biological systems, no gustatory mechanism to prevent ingestion of such material has been identified in any organism. We observed that light inhibits C. elegans feeding and used light as a tool to uncover molecular and neural mechanisms for gustation. Light can generate hydrogen peroxide, and we discovered that hydrogen peroxide similarly inhibits feeding. The gustatory receptor family members LITE-1 and GUR-3 are required for the inhibition of feeding by light and hydrogen peroxide. The I2 pharyngeal neurons increase calcium in response to light and hydrogen peroxide, and these responses require GUR-3 and a conserved antioxidant enzyme peroxiredoxin PRDX-2. Our results demonstrate a gustatory mechanism that mediates the detection and blocks ingestion of a noncanonical taste stimulus, hydrogen peroxide. National Science Foundation (U.S.). Graduate Research Fellowship Program National Institutes of Health (U.S.) (Grant GM24663) 2017-04-05T15:03:54Z 2017-04-05T15:03:54Z 2015-01 2014-11 Article http://purl.org/eprint/type/JournalArticle 0896-6273 1097-4199 http://hdl.handle.net/1721.1/107846 Bhatla, Nikhil, and H. Robert Horvitz. “Light and Hydrogen Peroxide Inhibit C. Elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons.” Neuron 85.4 (2015): 804–818. https://orcid.org/0000-0002-1693-4524 https://orcid.org/0000-0002-9964-9613 en_US http://dx.doi.org/10.1016/j.neuron.2014.12.061 Neuron Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier/Cell Press PMC |
| spellingShingle | Bhatla, Nikhil Horvitz, Howard Robert Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons |
| title | Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons |
| title_full | Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons |
| title_fullStr | Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons |
| title_full_unstemmed | Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons |
| title_short | Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons |
| title_sort | light and hydrogen peroxide inhibit c elegans feeding through gustatory receptor orthologs and pharyngeal neurons |
| url | http://hdl.handle.net/1721.1/107846 https://orcid.org/0000-0002-1693-4524 https://orcid.org/0000-0002-9964-9613 |
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