RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin
RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables...
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| Format: | Article |
| Language: | en_US |
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Springer New York
2017
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| Online Access: | http://hdl.handle.net/1721.1/108014 https://orcid.org/0000-0002-5754-1719 |
| _version_ | 1811086718389977088 |
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| author | Engreitz, Jesse Michael Lander, Eric Steven Guttman, Mitchell |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science Engreitz, Jesse Michael Lander, Eric Steven Guttman, Mitchell |
| author_sort | Engreitz, Jesse Michael |
| collection | MIT |
| description | RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing. |
| first_indexed | 2024-09-23T13:32:31Z |
| format | Article |
| id | mit-1721.1/108014 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:32:31Z |
| publishDate | 2017 |
| publisher | Springer New York |
| record_format | dspace |
| spelling | mit-1721.1/1080142022-09-28T14:40:00Z RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin Engreitz, Jesse Michael Lander, Eric Steven Guttman, Mitchell Institute for Medical Engineering and Science Massachusetts Institute of Technology. Department of Biology Lander, Eric Steven Engreitz, Jesse Michael Lander, Eric Steven Guttman, Mitchell RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing. 2017-04-10T17:20:21Z 2017-04-10T17:20:21Z 2015 Article http://purl.org/eprint/type/JournalArticle 1064-3745 1940-6029 http://hdl.handle.net/1721.1/108014 Engreitz, Jesse, Lander, Eric S., and Guttman, Mitchell. “RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.” Nuclear Bodies and Noncoding RNAs (December 5, 2014): 183–197. https://orcid.org/0000-0002-5754-1719 en_US http://dx.doi.org/10.1007/978-1-4939-2253-6_11 Nuclear Bodies and Noncoding RNAs Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer New York Prof. Lander via Courtney Crummett |
| spellingShingle | Engreitz, Jesse Michael Lander, Eric Steven Guttman, Mitchell RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin |
| title | RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin |
| title_full | RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin |
| title_fullStr | RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin |
| title_full_unstemmed | RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin |
| title_short | RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin |
| title_sort | rna antisense purification rap for mapping rna interactions with chromatin |
| url | http://hdl.handle.net/1721.1/108014 https://orcid.org/0000-0002-5754-1719 |
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