Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice
In this study, we examined colonization dynamics of segmented filamentous bacteria (SFB) in intestine of Swiss Webster (SW) mice infected with Helicobacter hepaticus (Hh). At 8 weeks post-inoculation with Hh (WPI), cecal and colonic SFB levels in the control males were significantly lower compared t...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2017
|
| Online Access: | http://hdl.handle.net/1721.1/108153 https://orcid.org/0000-0001-9307-6116 |
| _version_ | 1826190441192620032 |
|---|---|
| author | Ge, Zhongming Feng, Yan Woods, Stephanie Fox, James G |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ge, Zhongming Feng, Yan Woods, Stephanie Fox, James G |
| author_sort | Ge, Zhongming |
| collection | MIT |
| description | In this study, we examined colonization dynamics of segmented filamentous bacteria (SFB) in intestine of Swiss Webster (SW) mice infected with Helicobacter hepaticus (Hh). At 8 weeks post-inoculation with Hh (WPI), cecal and colonic SFB levels in the control males were significantly lower compared to those at 16 WPI. Hh infection in both genders did not alter SFB levels in the jejunum and ileum, but increased SFB levels in the cecum and colon of males compared to the controls (P < 0.05) at 8 WPI. At 16 WPI, the Hh-infected females contained lower levels of SFB in the jejunum, cecum and colon compared to the female controls. Irrespective of gender, aging and Hh infection, the Il-17A mRNA levels decreased from the small intestine to the cecum and then to the colon, whereas the Foxp3 mRNA levels were comparable in these intestinal regions. There were significant differences in Il-17A mRNA levels in the ileum (P < 0.05, R2 = 0.31), with females having greater Il-17A mRNA levels than males, and higher SFB colonization levels related to more Il-17A mRNA. These results indicate that aging and gender play an important role in colonization dynamics of intestinal SFB and ileal SFB-associated Th17 response. |
| first_indexed | 2024-09-23T08:40:17Z |
| format | Article |
| id | mit-1721.1/108153 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:40:17Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1081532022-09-30T10:24:41Z Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice Ge, Zhongming Feng, Yan Woods, Stephanie Fox, James G Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Division of Comparative Medicine Ge, Zhongming Feng, Yan Woods, Stephanie Fox, James G In this study, we examined colonization dynamics of segmented filamentous bacteria (SFB) in intestine of Swiss Webster (SW) mice infected with Helicobacter hepaticus (Hh). At 8 weeks post-inoculation with Hh (WPI), cecal and colonic SFB levels in the control males were significantly lower compared to those at 16 WPI. Hh infection in both genders did not alter SFB levels in the jejunum and ileum, but increased SFB levels in the cecum and colon of males compared to the controls (P < 0.05) at 8 WPI. At 16 WPI, the Hh-infected females contained lower levels of SFB in the jejunum, cecum and colon compared to the female controls. Irrespective of gender, aging and Hh infection, the Il-17A mRNA levels decreased from the small intestine to the cecum and then to the colon, whereas the Foxp3 mRNA levels were comparable in these intestinal regions. There were significant differences in Il-17A mRNA levels in the ileum (P < 0.05, R2 = 0.31), with females having greater Il-17A mRNA levels than males, and higher SFB colonization levels related to more Il-17A mRNA. These results indicate that aging and gender play an important role in colonization dynamics of intestinal SFB and ileal SFB-associated Th17 response. United States. National Institutes of Health (P30-ES002109) United States. National Institutes of Health (R01OD11141) United States. National Institutes of Health (R01-CA067529) 2017-04-13T21:42:14Z 2017-04-13T21:42:14Z 2014-10 2014-10 Article http://purl.org/eprint/type/JournalArticle 1286-4579 http://hdl.handle.net/1721.1/108153 Ge, Zhongming, Yan Feng, Stephanie E. Woods, and James G. Fox. “Spatial and Temporal Colonization Dynamics of Segmented Filamentous Bacteria Is Influenced by Gender, Age and Experimental Infection with Helicobacter Hepaticus in Swiss Webster Mice.” Microbes and Infection 17, no. 1 (January 2015): 16–22. https://orcid.org/0000-0001-9307-6116 en_US http://dx.doi.org/10.1016/j.micinf.2014.10.005 Microbes and Infection Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Ge, Zhongming Feng, Yan Woods, Stephanie Fox, James G Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice |
| title | Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice |
| title_full | Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice |
| title_fullStr | Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice |
| title_full_unstemmed | Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice |
| title_short | Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice |
| title_sort | spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender age and experimental infection with helicobacter hepaticus in swiss webster mice |
| url | http://hdl.handle.net/1721.1/108153 https://orcid.org/0000-0001-9307-6116 |
| work_keys_str_mv | AT gezhongming spatialandtemporalcolonizationdynamicsofsegmentedfilamentousbacteriaisinfluencedbygenderageandexperimentalinfectionwithhelicobacterhepaticusinswisswebstermice AT fengyan spatialandtemporalcolonizationdynamicsofsegmentedfilamentousbacteriaisinfluencedbygenderageandexperimentalinfectionwithhelicobacterhepaticusinswisswebstermice AT woodsstephanie spatialandtemporalcolonizationdynamicsofsegmentedfilamentousbacteriaisinfluencedbygenderageandexperimentalinfectionwithhelicobacterhepaticusinswisswebstermice AT foxjamesg spatialandtemporalcolonizationdynamicsofsegmentedfilamentousbacteriaisinfluencedbygenderageandexperimentalinfectionwithhelicobacterhepaticusinswisswebstermice |