Polymer Microarrays for High Throughput Discovery of Biomaterials
The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format. Herein is described the formation and characterization o...
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| Format: | Article |
| Language: | en_US |
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MyJoVE Corporation
2017
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| Online Access: | http://hdl.handle.net/1721.1/108248 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-5629-4798 |
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| author | Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. Langer, Robert S Anderson, Daniel Griffith |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. Langer, Robert S Anderson, Daniel Griffith |
| author_sort | Hook, Andrew L. |
| collection | MIT |
| description | The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format. Herein is described the formation and characterization of a polymer microarray using an on-chip photopolymerization technique. This involves mixing monomers at varied ratios to produce a library of monomer solutions, transferring the solution to a glass slide format using a robotic printing device and curing with UV irradiation. This format is readily amenable to many biological assays, including stem cell attachment and proliferation, cell sorting and low bacterial adhesion, allowing the ready identification of 'hit' materials that fulfill a specific biological criterion. Furthermore, the use of high throughput surface characterization (HTSC) allows the biological performance to be correlated with physio-chemical properties, hence elucidating the biological-material interaction. HTSC makes use of water contact angle (WCA) measurements, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). In particular, ToF-SIMS provides a chemically rich analysis of the sample that can be used to correlate the cell response with a molecular moiety. In some cases, the biological performance can be predicted from the ToF-SIMS spectra, demonstrating the chemical dependence of a biological-material interaction, and informing the development of hit materials. |
| first_indexed | 2024-09-23T11:14:04Z |
| format | Article |
| id | mit-1721.1/108248 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:14:04Z |
| publishDate | 2017 |
| publisher | MyJoVE Corporation |
| record_format | dspace |
| spelling | mit-1721.1/1082482022-10-01T02:16:00Z Polymer Microarrays for High Throughput Discovery of Biomaterials Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. Langer, Robert S Anderson, Daniel Griffith Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Langer, Robert S Anderson, Daniel Griffith The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format. Herein is described the formation and characterization of a polymer microarray using an on-chip photopolymerization technique. This involves mixing monomers at varied ratios to produce a library of monomer solutions, transferring the solution to a glass slide format using a robotic printing device and curing with UV irradiation. This format is readily amenable to many biological assays, including stem cell attachment and proliferation, cell sorting and low bacterial adhesion, allowing the ready identification of 'hit' materials that fulfill a specific biological criterion. Furthermore, the use of high throughput surface characterization (HTSC) allows the biological performance to be correlated with physio-chemical properties, hence elucidating the biological-material interaction. HTSC makes use of water contact angle (WCA) measurements, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). In particular, ToF-SIMS provides a chemically rich analysis of the sample that can be used to correlate the cell response with a molecular moiety. In some cases, the biological performance can be predicted from the ToF-SIMS spectra, demonstrating the chemical dependence of a biological-material interaction, and informing the development of hit materials. 2017-04-19T14:51:56Z 2017-04-19T14:51:56Z 2012-01 Article http://purl.org/eprint/type/JournalArticle 1940-087X http://hdl.handle.net/1721.1/108248 Hook, Andrew L., Chien-Yi Chang, Jing Yang, David J. Scurr, Robert Langer, Daniel G. Anderson, Steve Atkinson, Paul Williams, Martyn C. Davies, and Morgan R. Alexander. “Polymer Microarrays for High Throughput Discovery of Biomaterials.” Journal of Visualized Experiments no. 59 (January 25, 2012). https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-5629-4798 en_US http://dx.doi.org/10.3791/3636 Journal of Visualized Experiments Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/3.0/ application/pdf MyJoVE Corporation Journal of Visualized Experiments (JoVE) |
| spellingShingle | Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. Langer, Robert S Anderson, Daniel Griffith Polymer Microarrays for High Throughput Discovery of Biomaterials |
| title | Polymer Microarrays for High Throughput Discovery of Biomaterials |
| title_full | Polymer Microarrays for High Throughput Discovery of Biomaterials |
| title_fullStr | Polymer Microarrays for High Throughput Discovery of Biomaterials |
| title_full_unstemmed | Polymer Microarrays for High Throughput Discovery of Biomaterials |
| title_short | Polymer Microarrays for High Throughput Discovery of Biomaterials |
| title_sort | polymer microarrays for high throughput discovery of biomaterials |
| url | http://hdl.handle.net/1721.1/108248 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-5629-4798 |
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