A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase
Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2017
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| Online Access: | http://hdl.handle.net/1721.1/108280 https://orcid.org/0000-0002-8683-3593 https://orcid.org/0000-0001-8076-4489 |
| _version_ | 1826197035594809344 |
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| author | Wei, Yifeng Li, Bin Prakash, Divya Ferry, James G. Elliott, Sean J. Stubbe, JoAnne |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Wei, Yifeng Li, Bin Prakash, Divya Ferry, James G. Elliott, Sean J. Stubbe, JoAnne |
| author_sort | Wei, Yifeng |
| collection | MIT |
| description | Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism. |
| first_indexed | 2024-09-23T10:41:25Z |
| format | Article |
| id | mit-1721.1/108280 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:41:25Z |
| publishDate | 2017 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1082802022-09-30T22:19:20Z A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase Wei, Yifeng Li, Bin Prakash, Divya Ferry, James G. Elliott, Sean J. Stubbe, JoAnne Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Wei, Yifeng Stubbe, JoAnne Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism. Singapore. Agency for Science, Technology and Research National Institutes of Health (U.S.) (Grant GM081393) 2017-04-20T13:41:29Z 2017-04-20T13:41:29Z 2015-12 2015-10 Article http://purl.org/eprint/type/JournalArticle 0006-2960 1520-4995 http://hdl.handle.net/1721.1/108280 Wei, Yifeng et al. “A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina Barkeri Class III Ribonucleotide Reductase.” Biochemistry 54.47 (2015): 7019–7028. © 2015 American Chemical Society https://orcid.org/0000-0002-8683-3593 https://orcid.org/0000-0001-8076-4489 en_US http://dx.doi.org/10.1021/acs.biochem.5b01092 Biochemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Wei, Yifeng Li, Bin Prakash, Divya Ferry, James G. Elliott, Sean J. Stubbe, JoAnne A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase |
| title | A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase |
| title_full | A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase |
| title_fullStr | A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase |
| title_full_unstemmed | A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase |
| title_short | A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase |
| title_sort | ferredoxin disulfide reductase delivers electrons to the to the methanosarcina barkeri class iii ribonucleotide reductase |
| url | http://hdl.handle.net/1721.1/108280 https://orcid.org/0000-0002-8683-3593 https://orcid.org/0000-0001-8076-4489 |
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