Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa
Besides being a major opportunistic human pathogen, Pseudomonas aeruginosa can be found in a wide range of environments. This versatility is linked to complex regulation, which is achieved through the action of transcriptional regulators, and post-transcriptional regulation by intracellular protease...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/108282 |
| _version_ | 1826195424734609408 |
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| author | Fernández, Lucía Breidenstein, Elena B. M. Taylor, Patrick K. Bains, Manjeet Fang, Yuan Foster, Leonard J. Hancock, Robert E. W. de la Fuente Nunez, Cesar |
| author2 | MIT Synthetic Biology Center |
| author_facet | MIT Synthetic Biology Center Fernández, Lucía Breidenstein, Elena B. M. Taylor, Patrick K. Bains, Manjeet Fang, Yuan Foster, Leonard J. Hancock, Robert E. W. de la Fuente Nunez, Cesar |
| author_sort | Fernández, Lucía |
| collection | MIT |
| description | Besides being a major opportunistic human pathogen, Pseudomonas aeruginosa can be found in a wide range of environments. This versatility is linked to complex regulation, which is achieved through the action of transcriptional regulators, and post-transcriptional regulation by intracellular proteases including Lon. Indeed, lon mutants in this species show defects in motility, biofilm formation, pathogenicity and fluoroquinolone resistance. Here, the proteomic approach stable isotope labeling by amino acids in cell culture (SILAC) was used to search for novel proteolytic targets. One of the proteins that accumulated in the lon mutant was the RNA-binding protein Hfq. Further experiments demonstrated the ability of Lon to degrade Hfq in vitro. Also, overexpression of the hfq gene in the wild-type strain led to partial inhibition of swarming, swimming and twitching motilities, indicating that Hfq accumulation could contribute to the phenotypes displayed by Lon mutants. Hfq overexpression also led to the upregulation of the small regulatory RNA PhrS. Analysis of the phenotypes of strains lacking or overexpressing this sRNA indicated that the Lon protease might be indirectly regulating the levels and activity of sRNAs via Hfq. Overall, this study revealed new links in the complex regulatory chain that controls multicellular behaviours in P. aeruginosa. |
| first_indexed | 2024-09-23T10:12:26Z |
| format | Article |
| id | mit-1721.1/108282 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:12:26Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1082822022-09-30T19:39:10Z Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa Fernández, Lucía Breidenstein, Elena B. M. Taylor, Patrick K. Bains, Manjeet Fang, Yuan Foster, Leonard J. Hancock, Robert E. W. de la Fuente Nunez, Cesar MIT Synthetic Biology Center Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics de la Fuente Nunez, Cesar Besides being a major opportunistic human pathogen, Pseudomonas aeruginosa can be found in a wide range of environments. This versatility is linked to complex regulation, which is achieved through the action of transcriptional regulators, and post-transcriptional regulation by intracellular proteases including Lon. Indeed, lon mutants in this species show defects in motility, biofilm formation, pathogenicity and fluoroquinolone resistance. Here, the proteomic approach stable isotope labeling by amino acids in cell culture (SILAC) was used to search for novel proteolytic targets. One of the proteins that accumulated in the lon mutant was the RNA-binding protein Hfq. Further experiments demonstrated the ability of Lon to degrade Hfq in vitro. Also, overexpression of the hfq gene in the wild-type strain led to partial inhibition of swarming, swimming and twitching motilities, indicating that Hfq accumulation could contribute to the phenotypes displayed by Lon mutants. Hfq overexpression also led to the upregulation of the small regulatory RNA PhrS. Analysis of the phenotypes of strains lacking or overexpressing this sRNA indicated that the Lon protease might be indirectly regulating the levels and activity of sRNAs via Hfq. Overall, this study revealed new links in the complex regulatory chain that controls multicellular behaviours in P. aeruginosa. Fundación Obra Social de La Caixa Fundación Canadá Fundación Ramón Areces 2017-04-20T13:59:44Z 2017-04-20T13:59:44Z 2016-05 2015-12 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/108282 Fernández, Lucía et al. “Interconnection of Post-Transcriptional Regulation: The RNA-Binding Protein Hfq Is a Novel Target of the Lon Protease in Pseudomonas Aeruginosa.” Scientific Reports 6.1 (2016): n. pag. © 2017 Macmillan Publishers Limited en_US http://dx.doi.org/10.1038/srep26811 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Fernández, Lucía Breidenstein, Elena B. M. Taylor, Patrick K. Bains, Manjeet Fang, Yuan Foster, Leonard J. Hancock, Robert E. W. de la Fuente Nunez, Cesar Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa |
| title | Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa |
| title_full | Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa |
| title_fullStr | Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa |
| title_full_unstemmed | Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa |
| title_short | Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa |
| title_sort | interconnection of post transcriptional regulation the rna binding protein hfq is a novel target of the lon protease in pseudomonas aeruginosa |
| url | http://hdl.handle.net/1721.1/108282 |
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