Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip
Human red blood cells (RBCs) deformability in vitro was assessed during iron dextran (INFeD) loading and/or ethanol co-administration using microfluidic deformability screening. The results showed donor-specific variations in dose dependent deformability shift were revealed below 500 μg/mL iron dext...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/108299 https://orcid.org/0000-0003-3631-2148 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826215950205059072 |
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| author | Liu, Lihong Huang, Sha Xu, Xiaoying Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Liu, Lihong Huang, Sha Xu, Xiaoying Han, Jongyoon |
| author_sort | Liu, Lihong |
| collection | MIT |
| description | Human red blood cells (RBCs) deformability in vitro was assessed during iron dextran (INFeD) loading and/or ethanol co-administration using microfluidic deformability screening. The results showed donor-specific variations in dose dependent deformability shift were revealed below 500 μg/mL iron dextran. Two out of nine blood samples exhibited significant cell stiffening at 500 μg/mL iron dextran loading concentration (p < 0.05, Tukey test). More interestingly, co-administration of moderate amount of ethanol was identified to have significant protective effects on RBC deformability. We also noted that ethanol can reverse the deformability of impaired RBCs. Meanwhile obvious donor dependent response to ethanol administration on RBC deformability was noted using our biomimetic microfluidic chip. |
| first_indexed | 2024-09-23T16:40:04Z |
| format | Article |
| id | mit-1721.1/108299 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:40:04Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1082992022-09-29T20:39:00Z Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip Liu, Lihong Huang, Sha Xu, Xiaoying Han, Jongyoon Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Liu, Lihong Huang, Sha Han, Jongyoon Human red blood cells (RBCs) deformability in vitro was assessed during iron dextran (INFeD) loading and/or ethanol co-administration using microfluidic deformability screening. The results showed donor-specific variations in dose dependent deformability shift were revealed below 500 μg/mL iron dextran. Two out of nine blood samples exhibited significant cell stiffening at 500 μg/mL iron dextran loading concentration (p < 0.05, Tukey test). More interestingly, co-administration of moderate amount of ethanol was identified to have significant protective effects on RBC deformability. We also noted that ethanol can reverse the deformability of impaired RBCs. Meanwhile obvious donor dependent response to ethanol administration on RBC deformability was noted using our biomimetic microfluidic chip. Singapore-MIT Alliance in Research and Technology (SMART) 2017-04-20T17:42:50Z 2017-04-20T17:42:50Z 2016-03 2015-12 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/108299 Liu, Lihong, Sha Huang, Xiaoying Xu, and Jongyoon Han. “Study of Individual Erythrocyte Deformability Susceptibility to INFeD and Ethanol Using a Microfluidic Chip.” Scientific Reports 6 (March 11, 2016): 22929. © 2016 Macmillan Publishers Limited https://orcid.org/0000-0003-3631-2148 https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1038/srep22929 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Liu, Lihong Huang, Sha Xu, Xiaoying Han, Jongyoon Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip |
| title | Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip |
| title_full | Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip |
| title_fullStr | Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip |
| title_full_unstemmed | Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip |
| title_short | Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip |
| title_sort | study of individual erythrocyte deformability susceptibility to infed and ethanol using a microfluidic chip |
| url | http://hdl.handle.net/1721.1/108299 https://orcid.org/0000-0003-3631-2148 https://orcid.org/0000-0001-7215-1439 |
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