Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway
Cerebrospinal fluid (CSF) includes conserved factors whose function is largely unexplored. To assess the role of CSF during embryonic development, CSF was repeatedly drained from embryonic zebrafish brain ventricles soon after their inflation. Removal of CSF increased cell death in the diencephalon,...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2017
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| Online Access: | http://hdl.handle.net/1721.1/108349 https://orcid.org/0000-0002-4890-424X |
| _version_ | 1826209017725190144 |
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| author | Lehtinen, Maria K. Chang, Jessica T. Sive, Hazel L. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Lehtinen, Maria K. Chang, Jessica T. Sive, Hazel L. |
| author_sort | Lehtinen, Maria K. |
| collection | MIT |
| description | Cerebrospinal fluid (CSF) includes conserved factors whose function is largely unexplored. To assess the role of CSF during embryonic development, CSF was repeatedly drained from embryonic zebrafish brain ventricles soon after their inflation. Removal of CSF increased cell death in the diencephalon, indicating a survival function. Factors within the CSF are required for neuroepithelial cell survival as injected mouse CSF but not artificial CSF could prevent cell death after CSF depletion. Mass spectrometry analysis of the CSF identified retinol binding protein 4 (Rbp4), which transports retinol, the precursor to retinoic acid (RA). Consistent with a role for Rbp4 in cell survival, inhibition of Rbp4 or RA synthesis increased neuroepithelial cell death. Conversely, ventricle injection of exogenous human RBP4 plus retinol, or RA alone prevented cell death after CSF depletion. Zebrafish rbp4 is highly expressed in the yolk syncytial layer, suggesting Rbp4 protein and retinol/RA precursors can be transported into the CSF from the yolk. In accord with this suggestion, injection of human RBP4 protein into the yolk prevents neuroepithelial cell death in rbp4 loss-of-function embryos. Together, these data support the model that Rbp4 and RA precursors are present within the CSF and used for synthesis of RA, which promotes embryonic neuroepithelial survival. |
| first_indexed | 2024-09-23T14:16:22Z |
| format | Article |
| id | mit-1721.1/108349 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:16:22Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1083492022-09-28T19:39:57Z Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway Lehtinen, Maria K. Chang, Jessica T. Sive, Hazel L. Massachusetts Institute of Technology. Department of Biology Chang, Jessica Tzung-Min Sive, Hazel L Cerebrospinal fluid (CSF) includes conserved factors whose function is largely unexplored. To assess the role of CSF during embryonic development, CSF was repeatedly drained from embryonic zebrafish brain ventricles soon after their inflation. Removal of CSF increased cell death in the diencephalon, indicating a survival function. Factors within the CSF are required for neuroepithelial cell survival as injected mouse CSF but not artificial CSF could prevent cell death after CSF depletion. Mass spectrometry analysis of the CSF identified retinol binding protein 4 (Rbp4), which transports retinol, the precursor to retinoic acid (RA). Consistent with a role for Rbp4 in cell survival, inhibition of Rbp4 or RA synthesis increased neuroepithelial cell death. Conversely, ventricle injection of exogenous human RBP4 plus retinol, or RA alone prevented cell death after CSF depletion. Zebrafish rbp4 is highly expressed in the yolk syncytial layer, suggesting Rbp4 protein and retinol/RA precursors can be transported into the CSF from the yolk. In accord with this suggestion, injection of human RBP4 protein into the yolk prevents neuroepithelial cell death in rbp4 loss-of-function embryos. Together, these data support the model that Rbp4 and RA precursors are present within the CSF and used for synthesis of RA, which promotes embryonic neuroepithelial survival. 2017-04-21T17:30:51Z 2017-04-21T17:30:51Z 2015-05 2015-05 Article http://purl.org/eprint/type/JournalArticle 1932-8451 1932-846X http://hdl.handle.net/1721.1/108349 Chang, Jessica T.; Lehtinen, Maria K. and Sive, Hazel. “Zebrafish Cerebrospinal Fluid Mediates Cell Survival through a Retinoid Signaling Pathway.” Developmental Neurobiology 76, no. 1 (June 8, 2015): 75–92. © 2015 The Authors https://orcid.org/0000-0002-4890-424X en_US http://dx.doi.org/10.1002/dneu.22300 Developmental Neurobiology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Wiley Blackwell Wiley |
| spellingShingle | Lehtinen, Maria K. Chang, Jessica T. Sive, Hazel L. Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| title | Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| title_full | Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| title_fullStr | Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| title_full_unstemmed | Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| title_short | Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| title_sort | zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway |
| url | http://hdl.handle.net/1721.1/108349 https://orcid.org/0000-0002-4890-424X |
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