Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores
We use a suspended microchannel resonator to characterize the water and small-molecule permeability of Bacillus subtilis spores based on spores' buoyant mass in different solutions. Consistent with previous results, we found that the spore coat is not a significant barrier to small molecules, a...
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| 语言: | en_US |
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American Society for Microbiology
2017
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| 在线阅读: | http://hdl.handle.net/1721.1/108733 https://orcid.org/0000-0001-5277-6060 https://orcid.org/0000-0001-5223-9433 |
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| author | Setlow, Barbara Setlow, Peter Knudsen, Scott Cermak, Nathan Delgado, Francisco Feijo Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Setlow, Barbara Setlow, Peter Knudsen, Scott Cermak, Nathan Delgado, Francisco Feijo Manalis, Scott R |
| author_sort | Setlow, Barbara |
| collection | MIT |
| description | We use a suspended microchannel resonator to characterize the water and small-molecule permeability of Bacillus subtilis spores based on spores' buoyant mass in different solutions. Consistent with previous results, we found that the spore coat is not a significant barrier to small molecules, and the extent to which small molecules may enter the spore is size dependent. We have developed a method to directly observe the exchange kinetics of intraspore water with deuterium oxide, and we applied this method to wild-type spores and a panel of congenic mutants with deficiencies in the assembly or structure of the coat. Compared to wild-type spores, which exchange in approximately 1 s, several coat mutant spores were found to have relatively high water permeability with exchange times below the ∼200-ms temporal resolution of our assay. In addition, we found that the water permeability of the spore correlates with the ability of spores to germinate with dodecylamine and with the ability of TbCl₃ to inhibit germination with l-valine. These results suggest that the structure of the coat may be necessary for maintaining low water permeability. |
| first_indexed | 2024-09-23T10:15:53Z |
| format | Article |
| id | mit-1721.1/108733 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:15:53Z |
| publishDate | 2017 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | mit-1721.1/1087332022-09-30T20:02:33Z Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores Setlow, Barbara Setlow, Peter Knudsen, Scott Cermak, Nathan Delgado, Francisco Feijo Manalis, Scott R Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Manalis, Scott Knudsen, Scott Cermak, Nathan Delgado, Francisco Feijo Manalis, Scott R We use a suspended microchannel resonator to characterize the water and small-molecule permeability of Bacillus subtilis spores based on spores' buoyant mass in different solutions. Consistent with previous results, we found that the spore coat is not a significant barrier to small molecules, and the extent to which small molecules may enter the spore is size dependent. We have developed a method to directly observe the exchange kinetics of intraspore water with deuterium oxide, and we applied this method to wild-type spores and a panel of congenic mutants with deficiencies in the assembly or structure of the coat. Compared to wild-type spores, which exchange in approximately 1 s, several coat mutant spores were found to have relatively high water permeability with exchange times below the ∼200-ms temporal resolution of our assay. In addition, we found that the water permeability of the spore correlates with the ability of spores to germinate with dodecylamine and with the ability of TbCl₃ to inhibit germination with l-valine. These results suggest that the structure of the coat may be necessary for maintaining low water permeability. United States. Army Research Office (W911F-09-1-0286) United States. Army Research Office (W911NF-09-0001) 2017-05-08T14:18:28Z 2017-05-08T14:18:28Z 2015-10 2015-06 Article http://purl.org/eprint/type/JournalArticle 0021-9193 1098-5530 http://hdl.handle.net/1721.1/108733 Knudsen, Scott M.; Cermak, Nathan; Feijó Delgado, Francisco; Setlow, Barbara; Setlow, Peter and Manalis, Scott R. “Water and Small-Molecule Permeation of Dormant Bacillus Subtilis Spores.” Edited by P. de Boer. Journal of Bacteriology 198, no. 1 (October 2015): 168–177. https://orcid.org/0000-0001-5277-6060 https://orcid.org/0000-0001-5223-9433 en_US http://dx.doi.org/10.1128/jb.00435-15 Journal of Bacteriology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Society for Microbiology Prof. Manalis via Howard Silver |
| spellingShingle | Setlow, Barbara Setlow, Peter Knudsen, Scott Cermak, Nathan Delgado, Francisco Feijo Manalis, Scott R Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores |
| title | Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores |
| title_full | Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores |
| title_fullStr | Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores |
| title_full_unstemmed | Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores |
| title_short | Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores |
| title_sort | water and small molecule permeation of dormant bacillus subtilis spores |
| url | http://hdl.handle.net/1721.1/108733 https://orcid.org/0000-0001-5277-6060 https://orcid.org/0000-0001-5223-9433 |
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