Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment
We investigate recovery of multispecies oral biofilms following chlorhexidine gluconate (CHX) and CHX with surface modifiers (CHX-Plus) treatment. Specifically, we examine the percentage of viable bacteria in the biofilms following their exposure to CHX and CHX-Plus for 1, 3, and 10 minutes, respect...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/108754 |
| _version_ | 1826207963904212992 |
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| author | Shen, Ya Zhao, Jia Wang, Zhejun Hancock, Robert E. W. Roberts, Clive R. Ma, Jingzhi Li, Jun Haapasalo, Markus Wang, Qi de la Fuente Nunez, Cesar |
| author2 | Massachusetts Institute of Technology. Synthetic Biology Center |
| author_facet | Massachusetts Institute of Technology. Synthetic Biology Center Shen, Ya Zhao, Jia Wang, Zhejun Hancock, Robert E. W. Roberts, Clive R. Ma, Jingzhi Li, Jun Haapasalo, Markus Wang, Qi de la Fuente Nunez, Cesar |
| author_sort | Shen, Ya |
| collection | MIT |
| description | We investigate recovery of multispecies oral biofilms following chlorhexidine gluconate (CHX) and CHX with surface modifiers (CHX-Plus) treatment. Specifically, we examine the percentage of viable bacteria in the biofilms following their exposure to CHX and CHX-Plus for 1, 3, and 10 minutes, respectively. Before antimicrobial treatment, the biofilms are allowed to grow for three weeks. We find that (a). CHX-Plus kills bacteria in biofilms more effectively than the regular 2% CHX does, (b). cell continues to be killed for up to one week after exposure to the CHX solutions, (c). the biofilms start to recover after two weeks, the percentage of the viable bacteria recovers in the 1 and 3 minutes treatment groups but not in the 10 minutes treatment group after five weeks, and the biofilms fully return to the pretreatment levels after eight weeks. To understand the mechanism, a mathematical model for multiple bacterial phenotypes is developed, adopting the notion that bacterial persisters exist in the biofilms together with regulatory quorum sensing molecules and growth factor proteins. The model reveals the crucial role played by the persisters, quorum sensing molecules, and growth factors in biofilm recovery, accurately predicting the viable bacterial population after CHX treatment. |
| first_indexed | 2024-09-23T13:57:43Z |
| format | Article |
| id | mit-1721.1/108754 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:57:43Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1087542024-03-20T20:24:34Z Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment Shen, Ya Zhao, Jia Wang, Zhejun Hancock, Robert E. W. Roberts, Clive R. Ma, Jingzhi Li, Jun Haapasalo, Markus Wang, Qi de la Fuente Nunez, Cesar Massachusetts Institute of Technology. Synthetic Biology Center Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics de la Fuente Nunez, Cesar We investigate recovery of multispecies oral biofilms following chlorhexidine gluconate (CHX) and CHX with surface modifiers (CHX-Plus) treatment. Specifically, we examine the percentage of viable bacteria in the biofilms following their exposure to CHX and CHX-Plus for 1, 3, and 10 minutes, respectively. Before antimicrobial treatment, the biofilms are allowed to grow for three weeks. We find that (a). CHX-Plus kills bacteria in biofilms more effectively than the regular 2% CHX does, (b). cell continues to be killed for up to one week after exposure to the CHX solutions, (c). the biofilms start to recover after two weeks, the percentage of the viable bacteria recovers in the 1 and 3 minutes treatment groups but not in the 10 minutes treatment group after five weeks, and the biofilms fully return to the pretreatment levels after eight weeks. To understand the mechanism, a mathematical model for multiple bacterial phenotypes is developed, adopting the notion that bacterial persisters exist in the biofilms together with regulatory quorum sensing molecules and growth factor proteins. The model reveals the crucial role played by the persisters, quorum sensing molecules, and growth factors in biofilm recovery, accurately predicting the viable bacterial population after CHX treatment. Fundación Obra Social de La Caixa Fundación Canadá Fundación Ramón Areces (Postdoctoral Scholarship) 2017-05-08T19:11:28Z 2017-05-08T19:11:28Z 2016-06 2016-02 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/108754 Shen, Ya et al. “Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment.” Scientific Reports 6.1 (2016): n. pag. © 2017 Macmillan Publishers Limited en_US http://dx.doi.org/10.1038/srep27537 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Shen, Ya Zhao, Jia Wang, Zhejun Hancock, Robert E. W. Roberts, Clive R. Ma, Jingzhi Li, Jun Haapasalo, Markus Wang, Qi de la Fuente Nunez, Cesar Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment |
| title | Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment |
| title_full | Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment |
| title_fullStr | Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment |
| title_full_unstemmed | Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment |
| title_short | Experimental and Theoretical Investigation of Multispecies Oral Biofilm Resistance to Chlorhexidine Treatment |
| title_sort | experimental and theoretical investigation of multispecies oral biofilm resistance to chlorhexidine treatment |
| url | http://hdl.handle.net/1721.1/108754 |
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