Development and translation of label-free functional microscopy based on optical coherence tomography
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2017
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| Online Access: | http://hdl.handle.net/1721.1/108851 |
| _version_ | 1826189462493724672 |
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| author | Nam, Ahhyun |
| author2 | Benjamin J. Vakoc. |
| author_facet | Benjamin J. Vakoc. Nam, Ahhyun |
| author_sort | Nam, Ahhyun |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017. |
| first_indexed | 2024-09-23T08:15:28Z |
| format | Thesis |
| id | mit-1721.1/108851 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T08:15:28Z |
| publishDate | 2017 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1088512019-04-15T00:28:06Z Development and translation of label-free functional microscopy based on optical coherence tomography Nam, Ahhyun Benjamin J. Vakoc. Massachusetts Institute of Technology. Department of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering. Mechanical Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017. This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. Cataloged from student-submitted PDF version of thesis. Includes bibliographical references. Optical coherence tomography (OCT), an imaging modality based on low coherence interferometry, can be extended to obtain various endogenous functional contrasts. This thesis focuses on the development and translation of angiographic and polarization sensitive (PS) OCT techniques for clinical and preclinical applications. This goal includes four specific aims. The first aim is to develop a clinical imaging system to image the anatomy and microvasculature of human skin. The second aim is to develop a high performance post-processing algorithm for angiographic OCT. Towards this aim, we developed a processing algorithm based on complex differential variance (CDV) and confirmed its performance by benchmarking it against other published algorithms. The third aim is to develop a new approach for achieving high spatial resolution with an extended depth of focus for angiographic imaging. To achieve this aim, we have designed and built a triband wavelength system in which each spectrum is tightly focused at displaced focal planes to yield high transverse resolution over an effectively extended depth range. The fourth aim is to provide an imaging platform for preclinical study of peripheral nerve injury and repair. The vascularization is assessed by angiographic OCT, and the degree of myelination is measured by PS-OCT. These results confirm that the OCT platform can reveal new insights into preclinical studies of nerve regeneration and may ultimately provide a means for clinical intraoperative assessment of peripheral nerve health. by Ahhyun Stephanie Nam. Ph. D. 2017-05-11T19:09:28Z 2017-05-11T19:09:28Z 2017 2017 Thesis http://hdl.handle.net/1721.1/108851 986242577 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 167 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Nam, Ahhyun Development and translation of label-free functional microscopy based on optical coherence tomography |
| title | Development and translation of label-free functional microscopy based on optical coherence tomography |
| title_full | Development and translation of label-free functional microscopy based on optical coherence tomography |
| title_fullStr | Development and translation of label-free functional microscopy based on optical coherence tomography |
| title_full_unstemmed | Development and translation of label-free functional microscopy based on optical coherence tomography |
| title_short | Development and translation of label-free functional microscopy based on optical coherence tomography |
| title_sort | development and translation of label free functional microscopy based on optical coherence tomography |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/108851 |
| work_keys_str_mv | AT namahhyun developmentandtranslationoflabelfreefunctionalmicroscopybasedonopticalcoherencetomography |