RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology
Thesis: S.M., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2017.
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| Format: | Thèse |
| Langue: | eng |
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Massachusetts Institute of Technology
2017
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| Accès en ligne: | http://hdl.handle.net/1721.1/108981 |
| _version_ | 1826206926695825408 |
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| author | Palacios, Sebastian Ricardo |
| author2 | Ron Weiss. |
| author_facet | Ron Weiss. Palacios, Sebastian Ricardo |
| author_sort | Palacios, Sebastian Ricardo |
| collection | MIT |
| description | Thesis: S.M., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2017. |
| first_indexed | 2024-09-23T13:41:05Z |
| format | Thesis |
| id | mit-1721.1/108981 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:41:05Z |
| publishDate | 2017 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1089812019-04-11T13:04:52Z RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology Ribonucleic acid interference-based logic circuitry coupled to Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9-mediated transcriptional control in human cells and applications to stem cell technology Palacios, Sebastian Ricardo Ron Weiss. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis: S.M., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2017. Cataloged from PDF version of thesis. Includes bibliographical references (pages 37-38). This work demonstrates fundamental advancements towards the construction of an RNAi-based molecular computing core genetically encoded in the genome of human induced pluripotent stem cells (hiPSCs). This architecture can be theoretically programmed to precisely control stem cell differentiation. First, we use computational biology to analyze differential miRNA expression during human stem cell differentiation and guide biological design. We then design, build, and test proof-of-concept RNAi-based circuits in living human cells and couple them to Cas9-mediated transcriptional control. Finally, we demonstrate stable integration of an RNAi-based sensor in chromosome 19 (AAVS1 locus) of hiPSCs using landing pad technology followed by successful differentiation into brain-like tissue. by Sebastian Ricardo Palacios. S.M. 2017-05-11T19:59:00Z 2017-05-11T19:59:00Z 2017 2017 Thesis http://hdl.handle.net/1721.1/108981 986497452 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 38 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Palacios, Sebastian Ricardo RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology |
| title | RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology |
| title_full | RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology |
| title_fullStr | RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology |
| title_full_unstemmed | RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology |
| title_short | RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology |
| title_sort | rnai based logic circuitry coupled to cas9 mediated transcriptional control in human cells and applications to stem cell technology |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/108981 |
| work_keys_str_mv | AT palaciossebastianricardo rnaibasedlogiccircuitrycoupledtocas9mediatedtranscriptionalcontrolinhumancellsandapplicationstostemcelltechnology AT palaciossebastianricardo ribonucleicacidinterferencebasedlogiccircuitrycoupledtoclusteredregularlyinterspacedshortpalindromicrepeatsassociatedprotein9mediatedtranscriptionalcontrolinhumancellsandapplicationstostemcelltechnology |