Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care
Current biopharmaceutical manufacturing systems are not compatible with portable or distributed production of biologics, as they typically require the development of single biologic-producing cell lines followed by their cultivation at very large scales. Therefore, it remains challenging to treat pa...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/109076 https://orcid.org/0000-0003-4513-8575 https://orcid.org/0000-0002-5253-3365 https://orcid.org/0000-0002-2031-8871 https://orcid.org/0000-0003-0420-2235 https://orcid.org/0000-0002-9999-6690 |
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| author | Cleto, Sara Lee, Kevin Perez-Pinera, Pablo Han, Ningren Cao, Jicong Purcell, Oliver Shah, Kartik A Ram, Rajeev J Lu, Timothy K |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Cleto, Sara Lee, Kevin Perez-Pinera, Pablo Han, Ningren Cao, Jicong Purcell, Oliver Shah, Kartik A Ram, Rajeev J Lu, Timothy K |
| author_sort | Cleto, Sara |
| collection | MIT |
| description | Current biopharmaceutical manufacturing systems are not compatible with portable or distributed production of biologics, as they typically require the development of single biologic-producing cell lines followed by their cultivation at very large scales. Therefore, it remains challenging to treat patients in short time frames, especially in remote locations with limited infrastructure. To overcome these barriers, we developed a platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device. We use this platform for rapid and switchable production of two biologics from a single yeast strain as specified by the operator. Our results demonstrate selectable and near-single-dose production of these biologics in <24 h with limited infrastructure requirements. We envision that combining this system with analytical, purification and polishing technologies could lead to a small-scale, portable and fully integrated personal biomanufacturing platform that could advance disease treatment at point-of-care. |
| first_indexed | 2024-09-23T16:44:52Z |
| format | Article |
| id | mit-1721.1/109076 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:44:52Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1090762022-09-29T21:14:45Z Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care Cleto, Sara Lee, Kevin Perez-Pinera, Pablo Han, Ningren Cao, Jicong Purcell, Oliver Shah, Kartik A Ram, Rajeev J Lu, Timothy K Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Koch Institute for Integrative Cancer Research at MIT Perez-Pinera, Pablo Han, Ningren Cao, Jicong Purcell, Oliver Shah, Kartik A Ram, Rajeev J Lu, Timothy K Cleto, Sara Current biopharmaceutical manufacturing systems are not compatible with portable or distributed production of biologics, as they typically require the development of single biologic-producing cell lines followed by their cultivation at very large scales. Therefore, it remains challenging to treat patients in short time frames, especially in remote locations with limited infrastructure. To overcome these barriers, we developed a platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device. We use this platform for rapid and switchable production of two biologics from a single yeast strain as specified by the operator. Our results demonstrate selectable and near-single-dose production of these biologics in <24 h with limited infrastructure requirements. We envision that combining this system with analytical, purification and polishing technologies could lead to a small-scale, portable and fully integrated personal biomanufacturing platform that could advance disease treatment at point-of-care. 2017-05-15T13:51:01Z 2017-05-15T13:51:01Z 2016-07 2015-11 Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/109076 Perez-Pinera, Pablo; Han, Ningren; Cleto, Sara; Cao, Jicong; Purcell, Oliver; Shah, Kartik A.; Lee, Kevin; Ram, Rajeev and Lu, Timothy K. “Synthetic Biology and Microbioreactor Platforms for Programmable Production of Biologics at the Point-of-Care.” Nature Communications 7 (July 2016): 12211. © 2017 Macmillan Publishers Limited, part of Springer Nature https://orcid.org/0000-0003-4513-8575 https://orcid.org/0000-0002-5253-3365 https://orcid.org/0000-0002-2031-8871 https://orcid.org/0000-0003-0420-2235 https://orcid.org/0000-0002-9999-6690 en_US http://dx.doi.org/10.1038/ncomms12211 Nature Communications Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Cleto, Sara Lee, Kevin Perez-Pinera, Pablo Han, Ningren Cao, Jicong Purcell, Oliver Shah, Kartik A Ram, Rajeev J Lu, Timothy K Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care |
| title | Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care |
| title_full | Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care |
| title_fullStr | Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care |
| title_full_unstemmed | Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care |
| title_short | Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care |
| title_sort | synthetic biology and microbioreactor platforms for programmable production of biologics at the point of care |
| url | http://hdl.handle.net/1721.1/109076 https://orcid.org/0000-0003-4513-8575 https://orcid.org/0000-0002-5253-3365 https://orcid.org/0000-0002-2031-8871 https://orcid.org/0000-0003-0420-2235 https://orcid.org/0000-0002-9999-6690 |
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