Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/1721.1/109241 https://orcid.org/0000-0003-0705-0177 https://orcid.org/0000-0002-2026-8130 https://orcid.org/0000-0002-9387-8212 https://orcid.org/0000-0002-5560-8246 |
| _version_ | 1826215340655247360 |
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| author | Pardee, Keith Green, Alexander A. Lambert, Guillaume Ferrante, Tom Ma, Duo Donghia, Nina Fan, Melina Dudley, Dawn M. O’Connor, David H. Takahashi, Melissa Kimie Braff, Dana Lee, Jeongwook Daringer, Nichole Marie Bosch, Irene Gehrke, Lee Collins, James J. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Pardee, Keith Green, Alexander A. Lambert, Guillaume Ferrante, Tom Ma, Duo Donghia, Nina Fan, Melina Dudley, Dawn M. O’Connor, David H. Takahashi, Melissa Kimie Braff, Dana Lee, Jeongwook Daringer, Nichole Marie Bosch, Irene Gehrke, Lee Collins, James J. |
| author_sort | Pardee, Keith |
| collection | MIT |
| description | The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises. |
| first_indexed | 2024-09-23T16:24:33Z |
| format | Article |
| id | mit-1721.1/109241 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:24:33Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1092412022-09-29T19:48:41Z Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components Pardee, Keith Green, Alexander A. Lambert, Guillaume Ferrante, Tom Ma, Duo Donghia, Nina Fan, Melina Dudley, Dawn M. O’Connor, David H. Takahashi, Melissa Kimie Braff, Dana Lee, Jeongwook Daringer, Nichole Marie Bosch, Irene Gehrke, Lee Collins, James J. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Collins, James Takahashi, Melissa Kimie Braff, Dana Lee, Jeongwook Daringer, Nichole Marie Bosch, Irene Gehrke, Lee Collins, James J. The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises. Defense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006) United States. National Institutes of Health (NIH AI100190) 2017-05-22T13:39:41Z 2017-05-22T13:39:41Z 2016-05 2016-04 Article http://purl.org/eprint/type/JournalArticle 0092-8674 1097-4172 http://hdl.handle.net/1721.1/109241 Pardee, Keith; Green, Alexander A.; Takahashi, Melissa K.; Braff, Dana; Lambert, Guillaume; Lee, Jeong Wook; Ferrante, Tom et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell 165, no. 5 (May 2016): 1255–1266. © 2016 Elsevier Inc https://orcid.org/0000-0003-0705-0177 https://orcid.org/0000-0002-2026-8130 https://orcid.org/0000-0002-9387-8212 https://orcid.org/0000-0002-5560-8246 en_US http://dx.doi.org/10.1016/j.cell.2016.04.059 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Prof. Collins via Howard Silver |
| spellingShingle | Pardee, Keith Green, Alexander A. Lambert, Guillaume Ferrante, Tom Ma, Duo Donghia, Nina Fan, Melina Dudley, Dawn M. O’Connor, David H. Takahashi, Melissa Kimie Braff, Dana Lee, Jeongwook Daringer, Nichole Marie Bosch, Irene Gehrke, Lee Collins, James J. Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components |
| title | Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components |
| title_full | Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components |
| title_fullStr | Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components |
| title_full_unstemmed | Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components |
| title_short | Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components |
| title_sort | rapid low cost detection of zika virus using programmable biomolecular components |
| url | http://hdl.handle.net/1721.1/109241 https://orcid.org/0000-0003-0705-0177 https://orcid.org/0000-0002-2026-8130 https://orcid.org/0000-0002-9387-8212 https://orcid.org/0000-0002-5560-8246 |
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