Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study
Microfluidic synthesis of nanoparticles (NPs) can enhance the controllability and reproducibility in physicochemical properties of NPs compared to bulk synthesis methods. However, applications of microfluidic synthesis are typically limited to in vitro studies due to low production rates. Herein, we...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/1721.1/109289 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0003-0588-9286 |
| _version_ | 1826197944876924928 |
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| author | Farokhzad, Omid C. Lim, Jong-Min Bertrand, Nicolas Valencia, Pedro Miguel Rhee, Minsoung Langer, Robert S Jon, Sangyong Karnik, Rohit |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Farokhzad, Omid C. Lim, Jong-Min Bertrand, Nicolas Valencia, Pedro Miguel Rhee, Minsoung Langer, Robert S Jon, Sangyong Karnik, Rohit |
| author_sort | Farokhzad, Omid C. |
| collection | MIT |
| description | Microfluidic synthesis of nanoparticles (NPs) can enhance the controllability and reproducibility in physicochemical properties of NPs compared to bulk synthesis methods. However, applications of microfluidic synthesis are typically limited to in vitro studies due to low production rates. Herein, we report the parallelization of NP synthesis by 3D hydrodynamic flow focusing (HFF) using a multilayer microfluidic system to enhance the production rate without losing the advantages of reproducibility, controllability, and robustness. Using parallel 3D HFF, polymeric poly(lactide-co-glycolide)-b-polyethyleneglycol (PLGA-PEG) NPs with sizes tunable in the range of 13-150 nm could be synthesized reproducibly with high production rate. As a proof of concept, we used this system to perform in vivo pharmacokinetic and biodistribution study of small (20 nm diameter) PLGA-PEG NPs that are otherwise difficult to synthesize. Microfluidic parallelization thus enables synthesis of NPs with tunable properties with production rates suitable for both in vitro and in vivo studies. |
| first_indexed | 2024-09-23T10:56:33Z |
| format | Article |
| id | mit-1721.1/109289 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:56:33Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1092892022-10-01T00:05:17Z Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study Farokhzad, Omid C. Lim, Jong-Min Bertrand, Nicolas Valencia, Pedro Miguel Rhee, Minsoung Langer, Robert S Jon, Sangyong Karnik, Rohit Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Koch Institute for Integrative Cancer Research at MIT Lim, Jong-Min Bertrand, Nicolas Valencia, Pedro Miguel Rhee, Minsoung Langer, Robert S Jon, Sangyong Karnik, Rohit Microfluidic synthesis of nanoparticles (NPs) can enhance the controllability and reproducibility in physicochemical properties of NPs compared to bulk synthesis methods. However, applications of microfluidic synthesis are typically limited to in vitro studies due to low production rates. Herein, we report the parallelization of NP synthesis by 3D hydrodynamic flow focusing (HFF) using a multilayer microfluidic system to enhance the production rate without losing the advantages of reproducibility, controllability, and robustness. Using parallel 3D HFF, polymeric poly(lactide-co-glycolide)-b-polyethyleneglycol (PLGA-PEG) NPs with sizes tunable in the range of 13-150 nm could be synthesized reproducibly with high production rate. As a proof of concept, we used this system to perform in vivo pharmacokinetic and biodistribution study of small (20 nm diameter) PLGA-PEG NPs that are otherwise difficult to synthesize. Microfluidic parallelization thus enables synthesis of NPs with tunable properties with production rates suitable for both in vitro and in vivo studies. 2017-05-23T14:44:10Z 2017-05-23T14:44:10Z 2013-08 2013-06 Article http://purl.org/eprint/type/JournalArticle 1549-9634 http://hdl.handle.net/1721.1/109289 Lim, Jong-Min; Bertrand, Nicolas; Valencia, Pedro M.; Rhee, Minsoung; Langer, Robert; Jon, Sangyong; Farokhzad, Omid C. and Karnik, Rohit. “Parallel Microfluidic Synthesis of Size-Tunable Polymeric Nanoparticles Using 3D Flow Focusing Towards in Vivo Study.” Nanomedicine: Nanotechnology, Biology and Medicine 10, no. 2 (February 2014): 401–409 © 2014 Elsevier Inc https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0003-0588-9286 en_US http://dx.doi.org/10.1016/j.nano.2013.08.003 Nanomedicine: Nanotechnology, Biology and Medicine Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Farokhzad, Omid C. Lim, Jong-Min Bertrand, Nicolas Valencia, Pedro Miguel Rhee, Minsoung Langer, Robert S Jon, Sangyong Karnik, Rohit Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study |
| title | Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study |
| title_full | Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study |
| title_fullStr | Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study |
| title_full_unstemmed | Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study |
| title_short | Parallel microfluidic synthesis of size-tunable polymeric nanoparticles using 3D flow focusing towards in vivo study |
| title_sort | parallel microfluidic synthesis of size tunable polymeric nanoparticles using 3d flow focusing towards in vivo study |
| url | http://hdl.handle.net/1721.1/109289 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0003-0588-9286 |
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