Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds
Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/109340 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1811096666351075328 |
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| author | Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Nadkarni, Seemantini K. Grodzinsky, Alan J Jain, Rakesh Kumar |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Nadkarni, Seemantini K. Grodzinsky, Alan J Jain, Rakesh Kumar |
| author_sort | Hajjarian, Zeinab |
| collection | MIT |
| description | Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent laser beam illuminates the specimen and a high-speed camera acquires the time-varying speckle images. Cross-correlation analysis of frames returns the speckle intensity autocorrelation function, g[subscript 2](t), from which the frequency-dependent viscoelastic modulus, G*(ω), is deduced. Here, we establish the capability of LSR for evaluating the viscoelastic properties of hydrogels over a large range of moduli, using conventional mechanical rheometry and atomic force microscopy (AFM)-based indentation as reference-standards. Results demonstrate a strong correlation between |G*(ω)| values measured by LSR and mechanical rheometry (r = 0.95, p < 10−[superscript 9]), and z-test analysis reports that moduli values measured by the two methods are identical (p > 0.08) over a large range (47 Pa – 36 kPa). In addition, |G*(ω)| values measured by LSR correlate well with indentation moduli, E, reported by AFM (r = 0.92, p < 10−[superscript 7]). Further, spatially-resolved moduli measurements in micro-patterned substrates demonstrate that LSR combines the strengths of conventional rheology and micro-indentation in assessing hydrogel viscoelastic properties at multiple frequencies and small length-scales. |
| first_indexed | 2024-09-23T16:47:07Z |
| format | Article |
| id | mit-1721.1/109340 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:47:07Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1093402022-09-29T21:29:02Z Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Nadkarni, Seemantini K. Grodzinsky, Alan J Jain, Rakesh Kumar Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Center for Biomedical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Mechanical Engineering Grodzinsky, Alan J Jain, Rakesh Kumar Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent laser beam illuminates the specimen and a high-speed camera acquires the time-varying speckle images. Cross-correlation analysis of frames returns the speckle intensity autocorrelation function, g[subscript 2](t), from which the frequency-dependent viscoelastic modulus, G*(ω), is deduced. Here, we establish the capability of LSR for evaluating the viscoelastic properties of hydrogels over a large range of moduli, using conventional mechanical rheometry and atomic force microscopy (AFM)-based indentation as reference-standards. Results demonstrate a strong correlation between |G*(ω)| values measured by LSR and mechanical rheometry (r = 0.95, p < 10−[superscript 9]), and z-test analysis reports that moduli values measured by the two methods are identical (p > 0.08) over a large range (47 Pa – 36 kPa). In addition, |G*(ω)| values measured by LSR correlate well with indentation moduli, E, reported by AFM (r = 0.92, p < 10−[superscript 7]). Further, spatially-resolved moduli measurements in micro-patterned substrates demonstrate that LSR combines the strengths of conventional rheology and micro-indentation in assessing hydrogel viscoelastic properties at multiple frequencies and small length-scales. United States. National Institutes of Health (grant No. R01 HL 119867) 2017-05-25T15:49:06Z 2017-05-25T15:49:06Z 2016-12 2016-07 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/109340 Hajjarian, Zeinab, Hadi Tavakoli Nia, Shawn Ahn, Alan J. Grodzinsky, Rakesh K. Jain, and Seemantini K. Nadkarni. “Laser Speckle Rheology for Evaluating the Viscoelastic Properties of Hydrogel Scaffolds.” Scientific Reports 6, no. 1 (December 2016). https://orcid.org/0000-0002-4942-3456 en_US http://dx.doi.org/10.1038/srep37949 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Nadkarni, Seemantini K. Grodzinsky, Alan J Jain, Rakesh Kumar Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| title | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| title_full | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| title_fullStr | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| title_full_unstemmed | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| title_short | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| title_sort | laser speckle rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
| url | http://hdl.handle.net/1721.1/109340 https://orcid.org/0000-0002-4942-3456 |
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