Poly(Limonene Thioether) Scaffold for Tissue Engineering
A photocurable thiol-ene network polymer, poly(limonene thioether) (PLT32o), is synthesized, characterized, fabricated into tissue engineering scaffolds, and demonstrated in vitro and in vivo. Micromolded PLT32o grids exhibit compliant, elastomeric mechanical behavior similar to grids made of poly(g...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2017
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| Online Access: | http://hdl.handle.net/1721.1/109351 https://orcid.org/0000-0002-6750-2377 https://orcid.org/0000-0003-4144-6570 https://orcid.org/0000-0002-9101-4656 https://orcid.org/0000-0002-5585-9280 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0002-0720-8432 |
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| author | Fischer, Kristin McKeon Morgan, Kathy Ye Hearon II, Michael Keith Sklaviadis, Demetra Tochka, Zachary L Anderson, Daniel Griffith Langer, Robert S Freed, Lisa E Fenton, Owen Shea |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Fischer, Kristin McKeon Morgan, Kathy Ye Hearon II, Michael Keith Sklaviadis, Demetra Tochka, Zachary L Anderson, Daniel Griffith Langer, Robert S Freed, Lisa E Fenton, Owen Shea |
| author_sort | Fischer, Kristin McKeon |
| collection | MIT |
| description | A photocurable thiol-ene network polymer, poly(limonene thioether) (PLT32o), is synthesized, characterized, fabricated into tissue engineering scaffolds, and demonstrated in vitro and in vivo. Micromolded PLT32o grids exhibit compliant, elastomeric mechanical behavior similar to grids made of poly(glycerol sebacate) (PGS), an established biomaterial. Multilayered PL32o scaffolds with regular, geometrically defined pore architectures support heart cell seeding and culture in a manner similar to multilayered PGS scaffolds. Subcutaneous implantation of multilayered PLT32o scaffolds with cultured heart cells provides long-term 3D structural support and retains the exogenous cells, whereas PGS scaffolds lose both their structural integrity and the exogenous cells over 31 d in vivo. PLT32o membrane implants retain their dry mass, whereas PGS implants lose 70 percent of their dry mass by day 31. Macrophages are initially recruited to PLT32o and PGS membrane implants but are no longer present by day 31. Facile synthesis and processing in combination with the capability to support heart cells in vitro and in vivo suggest that PLT32o can offer advantages for tissue engineering applications where prolonged in vivo maintenance of 3D structural integrity and elastomeric mechanical behavior are required. |
| first_indexed | 2024-09-23T14:49:31Z |
| format | Article |
| id | mit-1721.1/109351 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:49:31Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1093512022-10-01T22:43:14Z Poly(Limonene Thioether) Scaffold for Tissue Engineering Fischer, Kristin McKeon Morgan, Kathy Ye Hearon II, Michael Keith Sklaviadis, Demetra Tochka, Zachary L Anderson, Daniel Griffith Langer, Robert S Freed, Lisa E Fenton, Owen Shea Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Koch Institute for Integrative Cancer Research at MIT Fischer, Kristin McKeon Morgan, Kathy Ye Hearon II, Michael Keith Sklaviadis, Demetra Tochka, Zachary L Fenton, Owen S. Anderson, Daniel Griffith Langer, Robert S Freed, Lisa E A photocurable thiol-ene network polymer, poly(limonene thioether) (PLT32o), is synthesized, characterized, fabricated into tissue engineering scaffolds, and demonstrated in vitro and in vivo. Micromolded PLT32o grids exhibit compliant, elastomeric mechanical behavior similar to grids made of poly(glycerol sebacate) (PGS), an established biomaterial. Multilayered PL32o scaffolds with regular, geometrically defined pore architectures support heart cell seeding and culture in a manner similar to multilayered PGS scaffolds. Subcutaneous implantation of multilayered PLT32o scaffolds with cultured heart cells provides long-term 3D structural support and retains the exogenous cells, whereas PGS scaffolds lose both their structural integrity and the exogenous cells over 31 d in vivo. PLT32o membrane implants retain their dry mass, whereas PGS implants lose 70 percent of their dry mass by day 31. Macrophages are initially recruited to PLT32o and PGS membrane implants but are no longer present by day 31. Facile synthesis and processing in combination with the capability to support heart cells in vitro and in vivo suggest that PLT32o can offer advantages for tissue engineering applications where prolonged in vivo maintenance of 3D structural integrity and elastomeric mechanical behavior are required. United States. National Institutes of Health (R01-HL107503) 2017-05-25T19:16:46Z 2017-05-25T19:16:46Z 2016-04 2015-11 Article http://purl.org/eprint/type/JournalArticle 2192-2640 http://hdl.handle.net/1721.1/109351 Fischer, Kristin M.; Morgan, Kathy Ye; Hearon, Keith; Sklaviadis, Demetra; Tochka, Zachary L.; Fenton, Owen S.; Anderson, Daniel G.; Langer, Robert and Freed, Lisa E.“Poly(Limonene Thioether) Scaffold for Tissue Engineering.” Advanced Healthcare Materials 5, no. 7 (February 2016): 813–821 © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim https://orcid.org/0000-0002-6750-2377 https://orcid.org/0000-0003-4144-6570 https://orcid.org/0000-0002-9101-4656 https://orcid.org/0000-0002-5585-9280 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0002-0720-8432 en_US http://dx.doi.org/10.1002/adhm.201500892 Advanced Healthcare Materials Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Fischer, Kristin McKeon Morgan, Kathy Ye Hearon II, Michael Keith Sklaviadis, Demetra Tochka, Zachary L Anderson, Daniel Griffith Langer, Robert S Freed, Lisa E Fenton, Owen Shea Poly(Limonene Thioether) Scaffold for Tissue Engineering |
| title | Poly(Limonene Thioether) Scaffold for Tissue Engineering |
| title_full | Poly(Limonene Thioether) Scaffold for Tissue Engineering |
| title_fullStr | Poly(Limonene Thioether) Scaffold for Tissue Engineering |
| title_full_unstemmed | Poly(Limonene Thioether) Scaffold for Tissue Engineering |
| title_short | Poly(Limonene Thioether) Scaffold for Tissue Engineering |
| title_sort | poly limonene thioether scaffold for tissue engineering |
| url | http://hdl.handle.net/1721.1/109351 https://orcid.org/0000-0002-6750-2377 https://orcid.org/0000-0003-4144-6570 https://orcid.org/0000-0002-9101-4656 https://orcid.org/0000-0002-5585-9280 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0002-0720-8432 |
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