Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

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In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it is difficult to perform this technique on frozen samples because freezing cells before extracting nuclei can impair nuclear integrity and alte...

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Bibliographic Details
Main Authors: Shelley, Brandon C., Mandefro, Berhan, Sareen, Dhruv, Svendsen, Clive N., Milani, Pamela, Escalante, Renan A., Patel-Murray, Natasha Leanna, Xin, Xiaofeng, Adam, Miriam, Fraenkel, Ernest
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program
Format: Article
Language:en_US
Published: Nature Publishing Group 2017
Online Access:http://hdl.handle.net/1721.1/109400
https://orcid.org/0000-0003-0250-0474
https://orcid.org/0000-0001-6913-4910
https://orcid.org/0000-0001-5230-3626
https://orcid.org/0000-0002-6804-251X
https://orcid.org/0000-0001-9249-8181

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