Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it is difficult to perform this technique on frozen samples because freezing cells before extracting nuclei can impair nuclear integrity and alte...

Full description

Bibliographic Details
Main Authors: Shelley, Brandon C., Mandefro, Berhan, Sareen, Dhruv, Svendsen, Clive N., Milani, Pamela, Escalante, Renan A., Patel-Murray, Natasha Leanna, Xin, Xiaofeng, Adam, Miriam, Fraenkel, Ernest
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program
Format: Article
Language:en_US
Published: Nature Publishing Group 2017
Online Access:http://hdl.handle.net/1721.1/109400
https://orcid.org/0000-0003-0250-0474
https://orcid.org/0000-0001-6913-4910
https://orcid.org/0000-0001-5230-3626
https://orcid.org/0000-0002-6804-251X
https://orcid.org/0000-0001-9249-8181