Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle
Microbes are an essential component of marine food webs and biogeochemical cycles, and therefore precise estimates of their biomass are of significant value. Here, we measured single-cell biomass distributions of isolates from several numerically abundant marine bacterial groups, including Pelagibac...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/109412 https://orcid.org/0000-0001-5277-6060 https://orcid.org/0000-0003-4564-3192 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-9296-3733 |
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| author | Cermak, Nathan Becker, Jamie William Knudsen, Scott Manalis, Scott R Polz, Martin F Chisholm, Sallie (Penny) |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Cermak, Nathan Becker, Jamie William Knudsen, Scott Manalis, Scott R Polz, Martin F Chisholm, Sallie (Penny) |
| author_sort | Cermak, Nathan |
| collection | MIT |
| description | Microbes are an essential component of marine food webs and biogeochemical cycles, and therefore precise estimates of their biomass are of significant value. Here, we measured single-cell biomass distributions of isolates from several numerically abundant marine bacterial groups, including Pelagibacter (SAR11), Prochlorococcus and Vibrio using a microfluidic mass sensor known as a suspended microchannel resonator (SMR). We show that the SMR can provide biomass (dry mass) measurements for cells spanning more than two orders of magnitude and that these estimates are consistent with other independent measures. We find that Pelagibacterales strain HTCC1062 has a median biomass of 11.9±0.7 fg per cell, which is five- to twelve-fold smaller than the median Prochlorococcus cell’s biomass (depending upon strain) and nearly 100-fold lower than that of rapidly growing V. splendidus strain 13B01. Knowing the biomass contributions from various taxonomic groups will provide more precise estimates of total marine biomass, aiding models of nutrient flux in the ocean. |
| first_indexed | 2024-09-23T14:54:53Z |
| format | Article |
| id | mit-1721.1/109412 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:54:53Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1094122022-10-01T23:18:24Z Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle Cermak, Nathan Becker, Jamie William Knudsen, Scott Manalis, Scott R Polz, Martin F Chisholm, Sallie (Penny) Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Manalis, Scott Cermak, Nathan Becker, Jamie William Knudsen, Scott Chisholm, Sallie W Manalis, Scott R Polz, Martin F Microbes are an essential component of marine food webs and biogeochemical cycles, and therefore precise estimates of their biomass are of significant value. Here, we measured single-cell biomass distributions of isolates from several numerically abundant marine bacterial groups, including Pelagibacter (SAR11), Prochlorococcus and Vibrio using a microfluidic mass sensor known as a suspended microchannel resonator (SMR). We show that the SMR can provide biomass (dry mass) measurements for cells spanning more than two orders of magnitude and that these estimates are consistent with other independent measures. We find that Pelagibacterales strain HTCC1062 has a median biomass of 11.9±0.7 fg per cell, which is five- to twelve-fold smaller than the median Prochlorococcus cell’s biomass (depending upon strain) and nearly 100-fold lower than that of rapidly growing V. splendidus strain 13B01. Knowing the biomass contributions from various taxonomic groups will provide more precise estimates of total marine biomass, aiding models of nutrient flux in the ocean. National Science Foundation (U.S.) (OCE-1129359) Simons Foundation (337262) United States. Army Research Office (W911NF-09-D-0001) 2017-05-30T15:23:41Z 2017-05-30T15:23:41Z 2016-12 2016-08 Article http://purl.org/eprint/type/JournalArticle 1751-7362 1751-7370 http://hdl.handle.net/1721.1/109412 Cermak, Nathan; Becker, Jamie W; Knudsen, Scott M; Chisholm, Sallie W; Manalis, Scott R and Polz, Martin F. “Direct Single-Cell Biomass Estimates for Marine Bacteria via Archimedes’ Principle.” The ISME Journal 11, no. 3 (December 2016): 825–828 © 2017 International Society for Microbial Ecology (ISME) https://orcid.org/0000-0001-5277-6060 https://orcid.org/0000-0003-4564-3192 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-9296-3733 en_US http://dx.doi.org/10.1038/ismej.2016.161 The ISME Journal Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Nature Publishing Group Prof. Manalis via Howard Silver |
| spellingShingle | Cermak, Nathan Becker, Jamie William Knudsen, Scott Manalis, Scott R Polz, Martin F Chisholm, Sallie (Penny) Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle |
| title | Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle |
| title_full | Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle |
| title_fullStr | Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle |
| title_full_unstemmed | Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle |
| title_short | Direct single-cell biomass estimates for marine bacteria via Archimedes’ principle |
| title_sort | direct single cell biomass estimates for marine bacteria via archimedes principle |
| url | http://hdl.handle.net/1721.1/109412 https://orcid.org/0000-0001-5277-6060 https://orcid.org/0000-0003-4564-3192 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-9296-3733 |
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