Engineering Stem Cell Organoids
Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactio...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2017
|
| Online Access: | http://hdl.handle.net/1721.1/109414 https://orcid.org/0000-0001-8624-8928 https://orcid.org/0000-0001-7258-7777 https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1826193749653323776 |
|---|---|
| author | Safaee, Helia Levy, Oren Yin, Xiaolei Mead, Benjamin Elliott Langer, Robert S Karp, Jeffrey Michael |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Safaee, Helia Levy, Oren Yin, Xiaolei Mead, Benjamin Elliott Langer, Robert S Karp, Jeffrey Michael |
| author_sort | Safaee, Helia |
| collection | MIT |
| description | Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies. |
| first_indexed | 2024-09-23T09:44:19Z |
| format | Article |
| id | mit-1721.1/109414 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:44:19Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1094142022-09-30T16:34:04Z Engineering Stem Cell Organoids Engineering Stem Cell Organoids Safaee, Helia Levy, Oren Yin, Xiaolei Mead, Benjamin Elliott Langer, Robert S Karp, Jeffrey Michael Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Yin, Xiaolei Mead, Benjamin Elliott Langer, Robert S Karp, Jeffrey Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies. United States. National Institutes of Health (HL095722) United States. National Institutes of Health (DE013023) 2017-05-30T15:43:02Z 2017-05-30T15:43:02Z 2016-01 Article http://purl.org/eprint/type/JournalArticle 1934-5909 http://hdl.handle.net/1721.1/109414 Yin, Xiaolei; Mead, Benjamin E.; Safaee, Helia; Langer, Robert; Karp, Jeffrey M. and Levy, Oren. “Engineering Stem Cell Organoids.” Cell Stem Cell 18, no. 1 (January 2016): 25–38 © 2016 Elsevier Inc https://orcid.org/0000-0001-8624-8928 https://orcid.org/0000-0001-7258-7777 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1016/j.stem.2015.12.005 Cell Stem Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Safaee, Helia Levy, Oren Yin, Xiaolei Mead, Benjamin Elliott Langer, Robert S Karp, Jeffrey Michael Engineering Stem Cell Organoids |
| title | Engineering Stem Cell Organoids |
| title_full | Engineering Stem Cell Organoids |
| title_fullStr | Engineering Stem Cell Organoids |
| title_full_unstemmed | Engineering Stem Cell Organoids |
| title_short | Engineering Stem Cell Organoids |
| title_sort | engineering stem cell organoids |
| url | http://hdl.handle.net/1721.1/109414 https://orcid.org/0000-0001-8624-8928 https://orcid.org/0000-0001-7258-7777 https://orcid.org/0000-0003-4255-0492 |
| work_keys_str_mv | AT safaeehelia engineeringstemcellorganoids AT levyoren engineeringstemcellorganoids AT yinxiaolei engineeringstemcellorganoids AT meadbenjaminelliott engineeringstemcellorganoids AT langerroberts engineeringstemcellorganoids AT karpjeffreymichael engineeringstemcellorganoids |