A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production
We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also al...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry, The
2017
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| Online Access: | http://hdl.handle.net/1721.1/109453 https://orcid.org/0000-0003-0420-2235 https://orcid.org/0000-0003-0921-3144 |
| _version_ | 1826194628563435520 |
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| author | Lee, Kevin S. Lee, Harry L. T. Mozdzierz, Nicholas Joseph Love, Kerry R. Shah, Kartik A Ram, Rajeev J Love, John C |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Innovation |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Innovation Lee, Kevin S. Lee, Harry L. T. Mozdzierz, Nicholas Joseph Love, Kerry R. Shah, Kartik A Ram, Rajeev J Love, John C |
| author_sort | Lee, Kevin S. |
| collection | MIT |
| description | We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing. |
| first_indexed | 2024-09-23T09:58:53Z |
| format | Article |
| id | mit-1721.1/109453 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:58:53Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/1094532022-09-26T14:59:54Z A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production Lee, Kevin S. Lee, Harry L. T. Mozdzierz, Nicholas Joseph Love, Kerry R. Shah, Kartik A Ram, Rajeev J Love, John C Massachusetts Institute of Technology. Center for Biomedical Innovation Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Research Laboratory of Electronics Koch Institute for Integrative Cancer Research at MIT Mozdzierz, Nicholas Joseph Love, Kerry R. Shah, Kartik A Ram, Rajeev J Love, John C We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing. United States. Defense Advanced Research Projects Agency (N66001-13-C-4025) National Cancer Institute (U.S.) (P30-CA14051) United States. National Institutes of Health (2T32GM008334-26) 2017-05-31T14:09:27Z 2017-05-31T14:09:27Z 2015-05 2015-04 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/109453 Mozdzierz, Nicholas J.; Love, Kerry R.; Lee, Kevin S.; Lee, Harry L. T.; Shah, Kartik A.; Ram, Rajeev J. and Love, J. Christopher. “A Perfusion-Capable Microfluidic Bioreactor for Assessing Microbial Heterologous Protein Production.” Lab on a Chip 15, no. 14 (May 2015): 2918–2922 © 2015 Royal Society of Chemistry https://orcid.org/0000-0003-0420-2235 https://orcid.org/0000-0003-0921-3144 en_US http://dx.doi.org/10.1039/c5lc00443h Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry, The PMC |
| spellingShingle | Lee, Kevin S. Lee, Harry L. T. Mozdzierz, Nicholas Joseph Love, Kerry R. Shah, Kartik A Ram, Rajeev J Love, John C A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production |
| title | A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production |
| title_full | A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production |
| title_fullStr | A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production |
| title_full_unstemmed | A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production |
| title_short | A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production |
| title_sort | perfusion capable microfluidic bioreactor for assessing microbial heterologous protein production |
| url | http://hdl.handle.net/1721.1/109453 https://orcid.org/0000-0003-0420-2235 https://orcid.org/0000-0003-0921-3144 |
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