In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography
Direct in vivo imaging of lymph flow is key to understanding lymphatic system function in normal and disease states. Optical microscopy techniques provide the resolution required for these measurements, but existing optical techniques for measuring lymph flow require complex protocols and provide li...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/109560 https://orcid.org/0000-0001-9714-0728 |
| _version_ | 1826204456639791104 |
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| author | Blatter, Cedric Meijer, Eelco F. J. Jones, Dennis Nam, Ahhyun Padera, Timothy P Bouma, Brett E Vakoc, Benjamin |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science Blatter, Cedric Meijer, Eelco F. J. Jones, Dennis Nam, Ahhyun Padera, Timothy P Bouma, Brett E Vakoc, Benjamin |
| author_sort | Blatter, Cedric |
| collection | MIT |
| description | Direct in vivo imaging of lymph flow is key to understanding lymphatic system function in normal and disease states. Optical microscopy techniques provide the resolution required for these measurements, but existing optical techniques for measuring lymph flow require complex protocols and provide limited temporal resolution. Here, we describe a Doppler optical coherence tomography platform that allows direct, label-free quantification of lymph velocity and volumetric flow rates. We overcome the challenge of very low scattering by employing a Doppler algorithm that operates on low signal-to-noise measurements. We show that this technique can measure lymph velocity at sufficiently high temporal resolution to resolve the dynamic pulsatile flow in collecting lymphatic vessels. |
| first_indexed | 2024-09-23T12:55:21Z |
| format | Article |
| id | mit-1721.1/109560 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:55:21Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1095602022-09-28T10:57:15Z In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography Blatter, Cedric Meijer, Eelco F. J. Jones, Dennis Nam, Ahhyun Padera, Timothy P Bouma, Brett E Vakoc, Benjamin Institute for Medical Engineering and Science Massachusetts Institute of Technology. Department of Mechanical Engineering Nam, Ahhyun Padera, Timothy P Bouma, Brett E Vakoc, Benjamin Direct in vivo imaging of lymph flow is key to understanding lymphatic system function in normal and disease states. Optical microscopy techniques provide the resolution required for these measurements, but existing optical techniques for measuring lymph flow require complex protocols and provide limited temporal resolution. Here, we describe a Doppler optical coherence tomography platform that allows direct, label-free quantification of lymph velocity and volumetric flow rates. We overcome the challenge of very low scattering by employing a Doppler algorithm that operates on low signal-to-noise measurements. We show that this technique can measure lymph velocity at sufficiently high temporal resolution to resolve the dynamic pulsatile flow in collecting lymphatic vessels. 2017-06-02T18:41:53Z 2017-06-02T18:41:53Z 2016-07 2016-02 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/109560 Blatter, Cedric; Meijer, Eelco F. J.; Nam, Ahhyun S.; Jones, Dennis; Bouma, Brett E.; Padera, Timothy P. and Vakoc, Benjamin J. “In Vivo Label-Free Measurement of Lymph Flow Velocity and Volumetric Flow Rates Using Doppler Optical Coherence Tomography.” Scientific Reports 6 (July 2016): 29035 © 2017 Macmillan Publishers Limited, part of Springer Nature https://orcid.org/0000-0001-9714-0728 en_US http://dx.doi.org/10.1038/srep29035 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Blatter, Cedric Meijer, Eelco F. J. Jones, Dennis Nam, Ahhyun Padera, Timothy P Bouma, Brett E Vakoc, Benjamin In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography |
| title | In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography |
| title_full | In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography |
| title_fullStr | In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography |
| title_full_unstemmed | In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography |
| title_short | In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography |
| title_sort | in vivo label free measurement of lymph flow velocity and volumetric flow rates using doppler optical coherence tomography |
| url | http://hdl.handle.net/1721.1/109560 https://orcid.org/0000-0001-9714-0728 |
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