Dynamic metabolic engineering: New strategies for developing responsive cell factories
Metabolic engineering strategies have enabled improvements in yield and titer for a variety of valuable small molecules produced naturally in microorganisms, as well as those produced via heterologous pathways. Typically, the approaches have been focused on up- and downregulation of genes to redistr...
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2017
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| Online Access: | http://hdl.handle.net/1721.1/109594 https://orcid.org/0000-0003-0585-2213 https://orcid.org/0000-0003-0437-3157 |
| _version_ | 1826197594618986496 |
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| author | Brockman, Irene Marie Jones, Kristala L. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Brockman, Irene Marie Jones, Kristala L. |
| author_sort | Brockman, Irene Marie |
| collection | MIT |
| description | Metabolic engineering strategies have enabled improvements in yield and titer for a variety of valuable small molecules produced naturally in microorganisms, as well as those produced via heterologous pathways. Typically, the approaches have been focused on up- and downregulation of genes to redistribute steady-state pathway fluxes, but more recently a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium. This review highlights some of the recently published work related to dynamic metabolic engineering strategies and explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems. Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates. The implementation is more complex relative to static control, but advances in screening techniques and DNA synthesis will continue to drive innovation in this field. |
| first_indexed | 2024-09-23T10:50:20Z |
| format | Article |
| id | mit-1721.1/109594 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:50:20Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1095942022-09-30T23:24:07Z Dynamic metabolic engineering: New strategies for developing responsive cell factories Brockman, Irene Marie Jones, Kristala L. Massachusetts Institute of Technology. Department of Chemical Engineering Brockman, Irene Marie Jones, Kristala L. Metabolic engineering strategies have enabled improvements in yield and titer for a variety of valuable small molecules produced naturally in microorganisms, as well as those produced via heterologous pathways. Typically, the approaches have been focused on up- and downregulation of genes to redistribute steady-state pathway fluxes, but more recently a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium. This review highlights some of the recently published work related to dynamic metabolic engineering strategies and explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems. Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates. The implementation is more complex relative to static control, but advances in screening techniques and DNA synthesis will continue to drive innovation in this field. National Science Foundation (U.S.) (CBET-0954986) United States. National Institutes of Health (T32GM008334) 2017-06-05T17:19:17Z 2017-06-05T17:19:17Z 2015-09 2015-02 Article http://purl.org/eprint/type/JournalArticle 1860-6768 1860-7314 http://hdl.handle.net/1721.1/109594 Brockman, Irene M. and Prather, Kristala L. J. “Dynamic Metabolic Engineering: New Strategies for Developing Responsive Cell Factories.” Biotechnology Journal 10, no. 9 (April 2015): 1360–1369 © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim https://orcid.org/0000-0003-0585-2213 https://orcid.org/0000-0003-0437-3157 en_US http://dx.doi.org/10.1002/biot.201400422 Biotechnology Journal Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Brockman, Irene Marie Jones, Kristala L. Dynamic metabolic engineering: New strategies for developing responsive cell factories |
| title | Dynamic metabolic engineering: New strategies for developing responsive cell factories |
| title_full | Dynamic metabolic engineering: New strategies for developing responsive cell factories |
| title_fullStr | Dynamic metabolic engineering: New strategies for developing responsive cell factories |
| title_full_unstemmed | Dynamic metabolic engineering: New strategies for developing responsive cell factories |
| title_short | Dynamic metabolic engineering: New strategies for developing responsive cell factories |
| title_sort | dynamic metabolic engineering new strategies for developing responsive cell factories |
| url | http://hdl.handle.net/1721.1/109594 https://orcid.org/0000-0003-0585-2213 https://orcid.org/0000-0003-0437-3157 |
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