Thermoresponsive and Mechanical Properties of Poly(
Gelation of the left helical N-substituted homopolypeptide poly(l-proline) (PLP) in water was explored, employing rheological and small-angle scattering studies at different temperatures and concentrations in order to investigate the network structure and its mechanical properties. Stiff gels were o...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2017
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| Online Access: | http://hdl.handle.net/1721.1/109602 https://orcid.org/0000-0002-7356-5489 https://orcid.org/0000-0002-0193-7378 https://orcid.org/0000-0002-7272-7140 |
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| author | Gkikas, Emmanouil Avery, Reginald Keith Olsen, Bradley D |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Gkikas, Emmanouil Avery, Reginald Keith Olsen, Bradley D |
| author_sort | Gkikas, Emmanouil |
| collection | MIT |
| description | Gelation of the left helical N-substituted homopolypeptide poly(l-proline) (PLP) in water was explored, employing rheological and small-angle scattering studies at different temperatures and concentrations in order to investigate the network structure and its mechanical properties. Stiff gels were obtained at 10 wt % or higher at 5 °C, the first time gelation has been observed for homopolypeptides. The secondary structure and helical rigidity of PLP has large structural similarities to gelatin but as gels the two materials show contrasting trends with temperature. With increasing temperature in D₂O, the network stiffens, with broad scattering features of similar correlation length for all concentrations and molar masses of PLP. A thermoresponsive transition was also achieved between 5 and 35 °C, with moduli at 35 °C higher than gelatin at 5 °C. The brittle gels could tolerate strains of 1% before yielding with a frequency-independent modulus over the observed range, similar to natural proline-rich proteins, suggesting the potential for thermoresponsive or biomaterial-based applications. |
| first_indexed | 2024-09-23T10:26:42Z |
| format | Article |
| id | mit-1721.1/109602 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:26:42Z |
| publishDate | 2017 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1096022022-09-26T17:57:50Z Thermoresponsive and Mechanical Properties of Poly( Gkikas, Emmanouil Avery, Reginald Keith Olsen, Bradley D Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Gkikas, Emmanouil Avery, Reginald Keith Olsen, Bradley D Gelation of the left helical N-substituted homopolypeptide poly(l-proline) (PLP) in water was explored, employing rheological and small-angle scattering studies at different temperatures and concentrations in order to investigate the network structure and its mechanical properties. Stiff gels were obtained at 10 wt % or higher at 5 °C, the first time gelation has been observed for homopolypeptides. The secondary structure and helical rigidity of PLP has large structural similarities to gelatin but as gels the two materials show contrasting trends with temperature. With increasing temperature in D₂O, the network stiffens, with broad scattering features of similar correlation length for all concentrations and molar masses of PLP. A thermoresponsive transition was also achieved between 5 and 35 °C, with moduli at 35 °C higher than gelatin at 5 °C. The brittle gels could tolerate strains of 1% before yielding with a frequency-independent modulus over the observed range, similar to natural proline-rich proteins, suggesting the potential for thermoresponsive or biomaterial-based applications. United States. Army Research Office (W911NF-13-D-0001) United States. National Institutes of Health (NIH/NIGMS 5T32GM008334) 2017-06-05T18:14:54Z 2017-06-05T18:14:54Z 2016-01 2015-08 Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 http://hdl.handle.net/1721.1/109602 Gkikas, Manos; Avery, Reginald K. and Olsen, Bradley D. “Thermoresponsive and Mechanical Properties of Poly(l-Proline) Gels.” Biomacromolecules 17, no. 2 (February 2016): 399–406 © 2016 American Chemical Society https://orcid.org/0000-0002-7356-5489 https://orcid.org/0000-0002-0193-7378 https://orcid.org/0000-0002-7272-7140 en_US http://dx.doi.org/10.1021/acs.biomac.5b01168 Biomacromolecules Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Gkikas, Emmanouil Avery, Reginald Keith Olsen, Bradley D Thermoresponsive and Mechanical Properties of Poly( |
| title | Thermoresponsive and Mechanical Properties of Poly( |
| title_full | Thermoresponsive and Mechanical Properties of Poly( |
| title_fullStr | Thermoresponsive and Mechanical Properties of Poly( |
| title_full_unstemmed | Thermoresponsive and Mechanical Properties of Poly( |
| title_short | Thermoresponsive and Mechanical Properties of Poly( |
| title_sort | thermoresponsive and mechanical properties of poly |
| url | http://hdl.handle.net/1721.1/109602 https://orcid.org/0000-0002-7356-5489 https://orcid.org/0000-0002-0193-7378 https://orcid.org/0000-0002-7272-7140 |
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