In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes
Detection of nitric oxide (NO) in vivo by single-walled carbon nanotubes (SWNT) is based on the fluorescent properties of SWNT and the ability of NO to quench the fluorescence signal. Alterations of the signal can be utilized to detect a small molecule in vivo that has not previously been possible b...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2017
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| Online Access: | http://hdl.handle.net/1721.1/109765 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0003-0771-9889 |
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| author | Iverson, Nicole M. Strano, Michael S. Wogan, Gerald N |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Iverson, Nicole M. Strano, Michael S. Wogan, Gerald N |
| author_sort | Iverson, Nicole M. |
| collection | MIT |
| description | Detection of nitric oxide (NO) in vivo by single-walled carbon nanotubes (SWNT) is based on the fluorescent properties of SWNT and the ability of NO to quench the fluorescence signal. Alterations of the signal can be utilized to detect a small molecule in vivo that has not previously been possible by other assay techniques. The protocols described here explain the techniques used to prepare NO-detecting SWNTs and to administer them to mice by both intravenous and subcutaneous routes. These techniques can also be utilized with other SWNT sensors as well as non-SWNT sensor |
| first_indexed | 2024-09-23T13:47:22Z |
| format | Article |
| id | mit-1721.1/109765 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:47:22Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1097652022-09-28T16:13:30Z In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes Iverson, Nicole M. Strano, Michael S. Wogan, Gerald N Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Iverson, Nicole M. Strano, Michael S. Wogan, Gerald N Detection of nitric oxide (NO) in vivo by single-walled carbon nanotubes (SWNT) is based on the fluorescent properties of SWNT and the ability of NO to quench the fluorescence signal. Alterations of the signal can be utilized to detect a small molecule in vivo that has not previously been possible by other assay techniques. The protocols described here explain the techniques used to prepare NO-detecting SWNTs and to administer them to mice by both intravenous and subcutaneous routes. These techniques can also be utilized with other SWNT sensors as well as non-SWNT sensor National Institutes of Health (T32 Training Grant in Environmental Toxicology ES007020) 2017-06-09T15:00:16Z 2017-06-09T15:00:16Z 2015-06 Article http://purl.org/eprint/type/JournalArticle http://hdl.handle.net/1721.1/109765 Iverson, Nicole M., Michael S. Strano, and Gerald N. Wogan. “In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes: NO-Sensing Single-Walled Carbon Nanotubes.” Current Protocols in Chemical Biology. Ed. Lara Mahal et al. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. 93–102. https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0003-0771-9889 en_US http://dx.doi.org/10.1002/9780470559277.ch140196 Current Protocols in Chemical Biology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Iverson, Nicole M. Strano, Michael S. Wogan, Gerald N In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes |
| title | In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes |
| title_full | In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes |
| title_fullStr | In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes |
| title_full_unstemmed | In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes |
| title_short | In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes |
| title_sort | in vivo delivery of nitric oxide sensing single walled carbon nanotubes |
| url | http://hdl.handle.net/1721.1/109765 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0003-0771-9889 |
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