Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants
Implantable, near infrared (nIR) fluorescent nanosensors are advantageous for in vivo monitoring of biological analytes since they can be rendered selective for particular target molecule while utilizing their unique optical properties and the nIR tissue transparency window for information transfer...
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| Format: | Article |
| Language: | en_US |
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American Scientific Publishers
2017
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| Online Access: | http://hdl.handle.net/1721.1/109767 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X |
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| author | Iverson, Nicole M. Bisker Raviv, Gili Hana Farias, Edgardo Ivanov, Vsevolod M Ahn, Jiyoung Wogan, Gerald N Strano, Michael S. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Iverson, Nicole M. Bisker Raviv, Gili Hana Farias, Edgardo Ivanov, Vsevolod M Ahn, Jiyoung Wogan, Gerald N Strano, Michael S. |
| author_sort | Iverson, Nicole M. |
| collection | MIT |
| description | Implantable, near infrared (nIR) fluorescent nanosensors are advantageous for in vivo monitoring of biological analytes since they can be rendered selective for particular target molecule while utilizing their unique optical properties and the nIR tissue transparency window for information transfer without an internal power source or telemetry. However, basic questions remain regarding the optimal encapsulation platform, geometrical properties, and concentration ranges required for
effective signal to noise ratio through biological tissue. In this work, we systematically explore these variables quantitatively to optimize the performance of such optical nanosensors for biomedical applications. We investigate both alginate and polyethylene glycol (PEG) as model
hydrogel systems, encapsulating d(GT)[subscript 15] ssDNA-wrapped single walled carbon nanotubes (SWNT) as model fluorescent nanoparticle sensors, responsive to riboflavin. Hydrogel sensors implanted 0.5 mm into thick tissue samples cause 50% reduction of initial fluorescence intensity,
allowing an optical detection limit of 5.4 mm and 5.1 mm depth in tissue for alginate and PEG gels, respectively, at a SWNT concentration of 10 mg L−1, and 785 nm laser excitation of 80 mW and 30 s exposure. These findings are supported with in vivo nIR fluorescent imaging of SWNT
hydrogels implanted subcutaneously in mice. For the case of SWNT, we find that the alginate system is preferable in terms of emission intensity, sensor response, rheological properties, and shelf life. |
| first_indexed | 2024-09-23T16:58:39Z |
| format | Article |
| id | mit-1721.1/109767 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:58:39Z |
| publishDate | 2017 |
| publisher | American Scientific Publishers |
| record_format | dspace |
| spelling | mit-1721.1/1097672022-10-03T09:34:00Z Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants Iverson, Nicole M. Bisker Raviv, Gili Hana Farias, Edgardo Ivanov, Vsevolod M Ahn, Jiyoung Wogan, Gerald N Strano, Michael S. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Physics Iverson, Nicole M. Bisker Raviv, Gili Hana Farias, Edgardo Ivanov, Vsevolod M Ahn, Jiyoung Wogan, Gerald N Strano, Michael S. Implantable, near infrared (nIR) fluorescent nanosensors are advantageous for in vivo monitoring of biological analytes since they can be rendered selective for particular target molecule while utilizing their unique optical properties and the nIR tissue transparency window for information transfer without an internal power source or telemetry. However, basic questions remain regarding the optimal encapsulation platform, geometrical properties, and concentration ranges required for effective signal to noise ratio through biological tissue. In this work, we systematically explore these variables quantitatively to optimize the performance of such optical nanosensors for biomedical applications. We investigate both alginate and polyethylene glycol (PEG) as model hydrogel systems, encapsulating d(GT)[subscript 15] ssDNA-wrapped single walled carbon nanotubes (SWNT) as model fluorescent nanoparticle sensors, responsive to riboflavin. Hydrogel sensors implanted 0.5 mm into thick tissue samples cause 50% reduction of initial fluorescence intensity, allowing an optical detection limit of 5.4 mm and 5.1 mm depth in tissue for alginate and PEG gels, respectively, at a SWNT concentration of 10 mg L−1, and 785 nm laser excitation of 80 mW and 30 s exposure. These findings are supported with in vivo nIR fluorescent imaging of SWNT hydrogels implanted subcutaneously in mice. For the case of SWNT, we find that the alginate system is preferable in terms of emission intensity, sensor response, rheological properties, and shelf life. National Institutes of Health (U.S.) (T32 Training Grant in Environmental Toxicology ES007020) National Cancer Institute (U.S.) (Grant P01 CA26731) National Institute of Environmental Health Sciences (Grant P30 ES002109) Arnold and Mabel Beckman Foundation (Young Investigator Award) National Science Foundation (U.S.) (Presidential Early Career Award for Scientists and Engineers) MIT-Technion Fellowship Samsung Scholarship Foundation Sanofi Aventis (Firm) (Biomedical Innovation Grant) 2017-06-09T15:31:12Z 2017-06-09T15:31:12Z 2016-05 Article http://purl.org/eprint/type/JournalArticle 1550-7033 http://hdl.handle.net/1721.1/109767 Iverson, Nicole M., Gili Bisker, Edgardo Farias, Vsevolod Ivanov, Jiyoung Ahn, Gerald N. Wogan, and Michael S. Strano. “Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants.” Journal of Biomedical Nanotechnology 12, no. 5 (May 1, 2016): 1035–1047. https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X en_US http://dx.doi.org/10.1166/jbn.2016.2237 Journal of Biomedical Nanotechnology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Scientific Publishers PMC |
| spellingShingle | Iverson, Nicole M. Bisker Raviv, Gili Hana Farias, Edgardo Ivanov, Vsevolod M Ahn, Jiyoung Wogan, Gerald N Strano, Michael S. Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants |
| title | Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants |
| title_full | Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants |
| title_fullStr | Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants |
| title_full_unstemmed | Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants |
| title_short | Quantitative Tissue Spectroscopy of Near Infrared Fluorescent Nanosensor Implants |
| title_sort | quantitative tissue spectroscopy of near infrared fluorescent nanosensor implants |
| url | http://hdl.handle.net/1721.1/109767 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X |
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