The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma

The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma

The development of sensitive and non-invasive “liquid biopsies” presents new opportunities for longitudinal monitoring of tumor dissemination and clonal evolution. The number of circulating tumor cells (CTCs) is prognostic in multiple myeloma (MM), but there is little information on their genetic fe...

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Bibliographic Details
Main Authors: Mishima, Yuji, Paiva, Bruno, Shi, Jiantao, Park, Jihye, Manier, Salomon, Takagi, Satoshi, Massoud, Mira, Perilla-Glen, Adriana, Aljawai, Yosra, Huynh, Daisy, Roccaro, Aldo M., Sacco, Antonio, Capelletti, Marzia, Detappe, Alexandre, Alignani, Diego, Anderson, Kenneth C., Munshi, Nikhil C., Prosper, Felipe, Lohr, Jens G., Ha, Gavin, Van Allen, Eliezer M., Michor, Franziska, San Miguel, Jesus F., Ghobrial, Irene M., Freeman, Samuel Spenser Sharpe, Adalsteinsson, Viktor A.
Other Authors: Broad Institute of MIT and Harvard
Format: Article
Language:en_US
Published: Elsevier 2017
Online Access:http://hdl.handle.net/1721.1/109832
https://orcid.org/0000-0003-4555-2485
Description
Summary:The development of sensitive and non-invasive “liquid biopsies” presents new opportunities for longitudinal monitoring of tumor dissemination and clonal evolution. The number of circulating tumor cells (CTCs) is prognostic in multiple myeloma (MM), but there is little information on their genetic features. Here, we have analyzed the genomic landscape of CTCs from 29 MM patients, including eight cases with matched/paired bone marrow (BM) tumor cells. Our results show that 100% of clonal mutations in patient BM were detected in CTCs and that 99% of clonal mutations in CTCs were present in BM MM. These include typical driver mutations in MM such as in KRAS, NRAS, or BRAF. These data suggest that BM and CTC samples have similar clonal structures, as discordances between the two were restricted to subclonal mutations. Accordingly, our results pave the way for potentially less invasive mutation screening of MM patients through characterization of CTCs.

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