The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma
The development of sensitive and non-invasive “liquid biopsies” presents new opportunities for longitudinal monitoring of tumor dissemination and clonal evolution. The number of circulating tumor cells (CTCs) is prognostic in multiple myeloma (MM), but there is little information on their genetic fe...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/1721.1/109832 https://orcid.org/0000-0003-4555-2485 |
| _version_ | 1826208388903600128 |
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| author | Mishima, Yuji Paiva, Bruno Shi, Jiantao Park, Jihye Manier, Salomon Takagi, Satoshi Massoud, Mira Perilla-Glen, Adriana Aljawai, Yosra Huynh, Daisy Roccaro, Aldo M. Sacco, Antonio Capelletti, Marzia Detappe, Alexandre Alignani, Diego Anderson, Kenneth C. Munshi, Nikhil C. Prosper, Felipe Lohr, Jens G. Ha, Gavin Van Allen, Eliezer M. Michor, Franziska San Miguel, Jesus F. Ghobrial, Irene M. Freeman, Samuel Spenser Sharpe Adalsteinsson, Viktor A. |
| author2 | Broad Institute of MIT and Harvard |
| author_facet | Broad Institute of MIT and Harvard Mishima, Yuji Paiva, Bruno Shi, Jiantao Park, Jihye Manier, Salomon Takagi, Satoshi Massoud, Mira Perilla-Glen, Adriana Aljawai, Yosra Huynh, Daisy Roccaro, Aldo M. Sacco, Antonio Capelletti, Marzia Detappe, Alexandre Alignani, Diego Anderson, Kenneth C. Munshi, Nikhil C. Prosper, Felipe Lohr, Jens G. Ha, Gavin Van Allen, Eliezer M. Michor, Franziska San Miguel, Jesus F. Ghobrial, Irene M. Freeman, Samuel Spenser Sharpe Adalsteinsson, Viktor A. |
| author_sort | Mishima, Yuji |
| collection | MIT |
| description | The development of sensitive and non-invasive “liquid biopsies” presents new opportunities for longitudinal monitoring of tumor dissemination and clonal evolution. The number of circulating tumor cells (CTCs) is prognostic in multiple myeloma (MM), but there is little information on their genetic features. Here, we have analyzed the genomic landscape of CTCs from 29 MM patients, including eight cases with matched/paired bone marrow (BM) tumor cells. Our results show that 100% of clonal mutations in patient BM were detected in CTCs and that 99% of clonal mutations in CTCs were present in BM MM. These include typical driver mutations in MM such as in KRAS, NRAS, or BRAF. These data suggest that BM and CTC samples have similar clonal structures, as discordances between the two were restricted to subclonal mutations. Accordingly, our results pave the way for potentially less invasive mutation screening of MM patients through characterization of CTCs. |
| first_indexed | 2024-09-23T14:04:59Z |
| format | Article |
| id | mit-1721.1/109832 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:04:59Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1098322022-10-01T19:05:18Z The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma Mishima, Yuji Paiva, Bruno Shi, Jiantao Park, Jihye Manier, Salomon Takagi, Satoshi Massoud, Mira Perilla-Glen, Adriana Aljawai, Yosra Huynh, Daisy Roccaro, Aldo M. Sacco, Antonio Capelletti, Marzia Detappe, Alexandre Alignani, Diego Anderson, Kenneth C. Munshi, Nikhil C. Prosper, Felipe Lohr, Jens G. Ha, Gavin Van Allen, Eliezer M. Michor, Franziska San Miguel, Jesus F. Ghobrial, Irene M. Freeman, Samuel Spenser Sharpe Adalsteinsson, Viktor A. Broad Institute of MIT and Harvard Freeman, Samuel Spenser Sharpe Adalsteinsson, Viktor A. The development of sensitive and non-invasive “liquid biopsies” presents new opportunities for longitudinal monitoring of tumor dissemination and clonal evolution. The number of circulating tumor cells (CTCs) is prognostic in multiple myeloma (MM), but there is little information on their genetic features. Here, we have analyzed the genomic landscape of CTCs from 29 MM patients, including eight cases with matched/paired bone marrow (BM) tumor cells. Our results show that 100% of clonal mutations in patient BM were detected in CTCs and that 99% of clonal mutations in CTCs were present in BM MM. These include typical driver mutations in MM such as in KRAS, NRAS, or BRAF. These data suggest that BM and CTC samples have similar clonal structures, as discordances between the two were restricted to subclonal mutations. Accordingly, our results pave the way for potentially less invasive mutation screening of MM patients through characterization of CTCs. United States. National Institutes of Health (R01 CA181683-01A1) American Association for Cancer Research (15-40-38-PAIV) 2017-06-13T18:55:34Z 2017-06-13T18:55:34Z 2017-04 Article http://purl.org/eprint/type/JournalArticle 2211-1247 http://hdl.handle.net/1721.1/109832 Mishima, Yuji; Paiva, Bruno; Shi, Jiantao; Park, Jihye; Manier, Salomon; Takagi, Satoshi; Massoud, Mira et al. “The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma.” Cell Reports 19, no. 1 (April 2017): 218–224 © 2017 The Authors https://orcid.org/0000-0003-4555-2485 en_US http://dx.doi.org/10.1016/j.celrep.2017.03.025 Cell Reports Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Elsevier |
| spellingShingle | Mishima, Yuji Paiva, Bruno Shi, Jiantao Park, Jihye Manier, Salomon Takagi, Satoshi Massoud, Mira Perilla-Glen, Adriana Aljawai, Yosra Huynh, Daisy Roccaro, Aldo M. Sacco, Antonio Capelletti, Marzia Detappe, Alexandre Alignani, Diego Anderson, Kenneth C. Munshi, Nikhil C. Prosper, Felipe Lohr, Jens G. Ha, Gavin Van Allen, Eliezer M. Michor, Franziska San Miguel, Jesus F. Ghobrial, Irene M. Freeman, Samuel Spenser Sharpe Adalsteinsson, Viktor A. The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma |
| title | The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma |
| title_full | The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma |
| title_fullStr | The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma |
| title_full_unstemmed | The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma |
| title_short | The Mutational Landscape of Circulating Tumor Cells in Multiple Myeloma |
| title_sort | mutational landscape of circulating tumor cells in multiple myeloma |
| url | http://hdl.handle.net/1721.1/109832 https://orcid.org/0000-0003-4555-2485 |
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