Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes
Despite significant advances in synthetic biology at industrial scales, digital fabrication challenges have, to date, precluded its implementation at the product scale. We present, Mushtari, a multimaterial 3D printed fluidic wearable designed to culture microbial communities. Thereby we introduce a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2017
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| Online Access: | http://hdl.handle.net/1721.1/109911 https://orcid.org/0000-0002-5628-5186 https://orcid.org/0000-0002-1465-4043 https://orcid.org/0000-0002-8775-5590 https://orcid.org/0000-0001-8822-7960 https://orcid.org/0000-0001-9222-4447 |
| _version_ | 1811075048388165632 |
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| author | Hays, Stephanie G. Dikovsky, Daniel Belocon, Boris Weaver, James C. Silver, Pamela A. Bader, Christoph Patrick, William Graham Kolb, Dominik Keating, Steven John Sharma, Sunanda Oxman, Neri |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Hays, Stephanie G. Dikovsky, Daniel Belocon, Boris Weaver, James C. Silver, Pamela A. Bader, Christoph Patrick, William Graham Kolb, Dominik Keating, Steven John Sharma, Sunanda Oxman, Neri |
| author_sort | Hays, Stephanie G. |
| collection | MIT |
| description | Despite significant advances in synthetic biology at industrial scales, digital fabrication challenges have, to date, precluded its implementation at the product scale. We present, Mushtari, a multimaterial 3D printed fluidic wearable designed to culture microbial communities. Thereby we introduce a computational design environment for additive manufacturing of geometrically complex and materially heterogeneous fluidic channels. We demonstrate how controlled variation of geometrical and optical properties at high spatial resolution can be achieved through a combination of computational growth modeling and multimaterial bitmap printing. Furthermore, we present the implementation, characterization, and evaluation of support methods for creating product-scale fluidics. Finally, we explore the cytotoxicity of 3D printed materials in culture studies with the model microorganisms, Escherichia coli and Bacillus subtilis. The results point toward design possibilities that lie at the intersection of computational design, additive manufacturing, and synthetic biology, with the ultimate goal of imparting biological functionality to 3D printed products. |
| first_indexed | 2024-09-23T09:59:49Z |
| format | Article |
| id | mit-1721.1/109911 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:59:49Z |
| publishDate | 2017 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/1099112022-09-26T15:05:16Z Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes Hays, Stephanie G. Dikovsky, Daniel Belocon, Boris Weaver, James C. Silver, Pamela A. Bader, Christoph Patrick, William Graham Kolb, Dominik Keating, Steven John Sharma, Sunanda Oxman, Neri Massachusetts Institute of Technology. Media Laboratory Bader, Christoph Patrick, William Graham Kolb, Dominik Keating, Steven John Sharma, Sunanda Oxman, Neri Despite significant advances in synthetic biology at industrial scales, digital fabrication challenges have, to date, precluded its implementation at the product scale. We present, Mushtari, a multimaterial 3D printed fluidic wearable designed to culture microbial communities. Thereby we introduce a computational design environment for additive manufacturing of geometrically complex and materially heterogeneous fluidic channels. We demonstrate how controlled variation of geometrical and optical properties at high spatial resolution can be achieved through a combination of computational growth modeling and multimaterial bitmap printing. Furthermore, we present the implementation, characterization, and evaluation of support methods for creating product-scale fluidics. Finally, we explore the cytotoxicity of 3D printed materials in culture studies with the model microorganisms, Escherichia coli and Bacillus subtilis. The results point toward design possibilities that lie at the intersection of computational design, additive manufacturing, and synthetic biology, with the ultimate goal of imparting biological functionality to 3D printed products. National Science Foundation (U.S.) (DGE1144152) United States. Department of Energy (DE-SC0012658) 2017-06-15T19:20:23Z 2017-06-15T19:20:23Z 2016-06 Article http://purl.org/eprint/type/JournalArticle 2329-7662 2329-7670 http://hdl.handle.net/1721.1/109911 Bader, Christoph; Patrick, William G.; Kolb, Dominik; Hays, Stephanie G.; Keating, Steven; Sharma, Sunanda; Dikovsky, Daniel et al. “Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes.” 3D Printing and Additive Manufacturing 3, no. 2 (June 2016): 79–89 © 2016 Mary Ann Liebert, Inc https://orcid.org/0000-0002-5628-5186 https://orcid.org/0000-0002-1465-4043 https://orcid.org/0000-0002-8775-5590 https://orcid.org/0000-0001-8822-7960 https://orcid.org/0000-0001-9222-4447 en_US http://dx.doi.org/10.1089/3dp.2016.0027 3D Printing and Additive Manufacturing Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Leibert |
| spellingShingle | Hays, Stephanie G. Dikovsky, Daniel Belocon, Boris Weaver, James C. Silver, Pamela A. Bader, Christoph Patrick, William Graham Kolb, Dominik Keating, Steven John Sharma, Sunanda Oxman, Neri Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes |
| title | Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes |
| title_full | Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes |
| title_fullStr | Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes |
| title_full_unstemmed | Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes |
| title_short | Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes |
| title_sort | grown printed and biologically augmented an additively manufactured microfluidic wearable functionally templated for synthetic microbes |
| url | http://hdl.handle.net/1721.1/109911 https://orcid.org/0000-0002-5628-5186 https://orcid.org/0000-0002-1465-4043 https://orcid.org/0000-0002-8775-5590 https://orcid.org/0000-0001-8822-7960 https://orcid.org/0000-0001-9222-4447 |
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