Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes
Molecular characterization of organ-specific metastatic lesions, which distinguish them from the primary tumor, will provide a better understanding of tissue specific adaptations that regulate metastatic progression. Using an orthotopic xenograft model, we have isolated isogenic metastatic human bre...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Impact Journals/National Center for Biotechnology Information (U.S.)
2017
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| Online Access: | http://hdl.handle.net/1721.1/109939 |
| _version_ | 1826201961841557504 |
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| author | Winnard Jr., Paul T. Zhang, Chi Vesuna, Farhad Garry, Jonah Barman, Ishan Raman, Venu Kang, Jeon Woong Dasari, Ramachandra Rao |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Winnard Jr., Paul T. Zhang, Chi Vesuna, Farhad Garry, Jonah Barman, Ishan Raman, Venu Kang, Jeon Woong Dasari, Ramachandra Rao |
| author_sort | Winnard Jr., Paul T. |
| collection | MIT |
| description | Molecular characterization of organ-specific metastatic lesions, which distinguish them from the primary tumor, will provide a better understanding of tissue specific adaptations that regulate metastatic progression. Using an orthotopic xenograft model, we have isolated isogenic metastatic human breast cancer cell lines directly from organ explants that are phenotypically distinct from the primary tumor cell line. Label-free Raman spectroscopy was used and informative spectral bands were ascertained as differentiators of organ-specific metastases as opposed to the presence of a single universal marker. Decision algorithms derived from the Raman spectra unambiguously identified these isogenic cell lines as unique biological entities – a finding reinforced through metabolomic analyses that indicated tissue of origin metabolite distinctions between the cell lines. Notably, complementarity of the metabolomics and Raman datasets was found. Our findings provide evidence that metastatic spread generates tissue-specific adaptations at the molecular level within cancer cells, which can be differentiated with Raman spectroscopy. |
| first_indexed | 2024-09-23T11:59:44Z |
| format | Article |
| id | mit-1721.1/109939 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:59:44Z |
| publishDate | 2017 |
| publisher | Impact Journals/National Center for Biotechnology Information (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1099392022-09-27T23:23:20Z Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes Winnard Jr., Paul T. Zhang, Chi Vesuna, Farhad Garry, Jonah Barman, Ishan Raman, Venu Kang, Jeon Woong Dasari, Ramachandra Rao Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Laser Biomedical Research Center Kang, Jeon Woong Dasari, Ramachandra Rao Molecular characterization of organ-specific metastatic lesions, which distinguish them from the primary tumor, will provide a better understanding of tissue specific adaptations that regulate metastatic progression. Using an orthotopic xenograft model, we have isolated isogenic metastatic human breast cancer cell lines directly from organ explants that are phenotypically distinct from the primary tumor cell line. Label-free Raman spectroscopy was used and informative spectral bands were ascertained as differentiators of organ-specific metastases as opposed to the presence of a single universal marker. Decision algorithms derived from the Raman spectra unambiguously identified these isogenic cell lines as unique biological entities – a finding reinforced through metabolomic analyses that indicated tissue of origin metabolite distinctions between the cell lines. Notably, complementarity of the metabolomics and Raman datasets was found. Our findings provide evidence that metastatic spread generates tissue-specific adaptations at the molecular level within cancer cells, which can be differentiated with Raman spectroscopy. National Institute of Biomedical Imaging and Bioengineering (U.S.) (9P41EB015871-26A1) 2017-06-16T14:18:07Z 2017-06-16T14:18:07Z 2017-01 2015-12 Article http://purl.org/eprint/type/JournalArticle 1949-2553 http://hdl.handle.net/1721.1/109939 Winnard Jr., Paul T. et al. “Organ-Specific Isogenic Metastatic Breast Cancer Cell Lines Exhibit Distinct Raman Spectral Signatures and Metabolomes.” Oncotarget (2017): n. pag. en_US http://dx.doi.org/10.18632/oncotarget.14865 Oncotarget Creative Commons Attribution 3.0 Unported license http://creativecommons.org/licenses/by/3.0/ application/pdf Impact Journals/National Center for Biotechnology Information (U.S.) Impact Journals |
| spellingShingle | Winnard Jr., Paul T. Zhang, Chi Vesuna, Farhad Garry, Jonah Barman, Ishan Raman, Venu Kang, Jeon Woong Dasari, Ramachandra Rao Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes |
| title | Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes |
| title_full | Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes |
| title_fullStr | Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes |
| title_full_unstemmed | Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes |
| title_short | Organ-specific isogenic metastatic breast cancer cell lines exhibit distinct Raman spectral signatures and metabolomes |
| title_sort | organ specific isogenic metastatic breast cancer cell lines exhibit distinct raman spectral signatures and metabolomes |
| url | http://hdl.handle.net/1721.1/109939 |
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