3D-Printable Materials for Microbial Liquid Culture
Additive manufacturing is providing the opportunity to easily fabricate complex structures for biological applications, such as growing microbial cultures. To best determine 3D-printable materials suitable for liquid microbial growth, culture tubes were printed that mimic the shape and dimensions o...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Mary Ann Liebert, Inc.
2017
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| Online Access: | http://hdl.handle.net/1721.1/109946 |
| _version_ | 1811084904263319552 |
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| author | Sorensen, Morgan T. Walsh, Matthew E. Ostrinskaya, Alla Kong, David S Carr Sr, Peter A. |
| author2 | Lincoln Laboratory |
| author_facet | Lincoln Laboratory Sorensen, Morgan T. Walsh, Matthew E. Ostrinskaya, Alla Kong, David S Carr Sr, Peter A. |
| author_sort | Sorensen, Morgan T. |
| collection | MIT |
| description | Additive manufacturing is providing the opportunity to easily fabricate complex structures for biological applications, such as growing microbial cultures. To best determine 3D-printable materials suitable for liquid
microbial growth, culture tubes were printed that mimic the shape and dimensions of a standard commercial tube. Ten different printed materials were assessed in conjunction with multiple 3D printing platforms, and their
performance to standard commercial polystyrene culture tubes was compared. In each test, a K-12 strain of the common laboratory bacterium Escherichia coli was monitored for growth over 24 h under normal conditions of temperature and agitation. Growth inhibition was observed for some printed materials, and mass spectrometry was used to identify two leached chemicals that inhibited bacterial growth. Based on this assessment, three of the materials are recommended for consideration in prototyping 3D-printed culture environments. |
| first_indexed | 2024-09-23T12:59:27Z |
| format | Article |
| id | mit-1721.1/109946 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:59:27Z |
| publishDate | 2017 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/1099462022-09-28T11:19:46Z 3D-Printable Materials for Microbial Liquid Culture Sorensen, Morgan T. Walsh, Matthew E. Ostrinskaya, Alla Kong, David S Carr Sr, Peter A. Lincoln Laboratory Massachusetts Institute of Technology. Media Laboratory Walsh, Matthew E. Ostrinskaya, Alla Kong, David S Carr Sr, Peter A. Additive manufacturing is providing the opportunity to easily fabricate complex structures for biological applications, such as growing microbial cultures. To best determine 3D-printable materials suitable for liquid microbial growth, culture tubes were printed that mimic the shape and dimensions of a standard commercial tube. Ten different printed materials were assessed in conjunction with multiple 3D printing platforms, and their performance to standard commercial polystyrene culture tubes was compared. In each test, a K-12 strain of the common laboratory bacterium Escherichia coli was monitored for growth over 24 h under normal conditions of temperature and agitation. Growth inhibition was observed for some printed materials, and mass spectrometry was used to identify two leached chemicals that inhibited bacterial growth. Based on this assessment, three of the materials are recommended for consideration in prototyping 3D-printed culture environments. Office of the Assistant Secretary of Defense for Research and Engineering (Contract FA8721-05-C-0002) 2017-06-16T14:59:14Z 2017-06-16T14:59:14Z 2016-06 Article http://purl.org/eprint/type/JournalArticle 2329-7662 2329-7670 http://hdl.handle.net/1721.1/109946 Walsh, Matthew E. et al. “3D-Printable Materials for Microbial Liquid Culture.” 3D Printing and Additive Manufacturing 3.2 (2016): 113–118. en_US http://dx.doi.org/10.1089/3dp.2016.0007 3D Printing and Additive Manufacturing Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Mary Ann Liebert, Inc. Mary Ann Liebert |
| spellingShingle | Sorensen, Morgan T. Walsh, Matthew E. Ostrinskaya, Alla Kong, David S Carr Sr, Peter A. 3D-Printable Materials for Microbial Liquid Culture |
| title | 3D-Printable Materials for Microbial Liquid Culture |
| title_full | 3D-Printable Materials for Microbial Liquid Culture |
| title_fullStr | 3D-Printable Materials for Microbial Liquid Culture |
| title_full_unstemmed | 3D-Printable Materials for Microbial Liquid Culture |
| title_short | 3D-Printable Materials for Microbial Liquid Culture |
| title_sort | 3d printable materials for microbial liquid culture |
| url | http://hdl.handle.net/1721.1/109946 |
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