Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice
Helicobacter pylori (H.pylori), a bacterial pathogen, is a causative agent of gastritis and peptic ulcer disease and is a strong risk factor for development of gastric cancer. Environmental conditions, such as poor dietary iron resulting in iron deficiency anemia (IDA), enhance H.pylori virulence an...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2017
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| Online Access: | http://hdl.handle.net/1721.1/109954 https://orcid.org/0000-0002-3418-056X https://orcid.org/0000-0001-9307-6116 |
| _version_ | 1811074175342739456 |
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| author | Amaya, Aldo Ennis, Kathleen Wang, Timothy C. Georgieff, Michael Burns, Monika Bodi Winn, Caroline M Ge, Zhongming Bakthavatchalu, Vasudevan Fox, James G |
| author2 | Broad Institute of MIT and Harvard |
| author_facet | Broad Institute of MIT and Harvard Amaya, Aldo Ennis, Kathleen Wang, Timothy C. Georgieff, Michael Burns, Monika Bodi Winn, Caroline M Ge, Zhongming Bakthavatchalu, Vasudevan Fox, James G |
| author_sort | Amaya, Aldo |
| collection | MIT |
| description | Helicobacter pylori (H.pylori), a bacterial pathogen, is a causative agent of gastritis and peptic ulcer disease and is a strong risk factor for development of gastric cancer. Environmental conditions, such as poor dietary iron resulting in iron deficiency anemia (IDA), enhance H.pylori virulence and increases risk for gastric cancer. IDA affects billions of people worldwide, and there is considerable overlap between regions of high IDA and high H.pylori prevalence. The primary aims of our study were to evaluate the effect of H.pylori infection on behavior, iron metabolism, red blood cell indices, and behavioral outcomes following comorbid H. pylori infection and dietary iron deficiency in a mouse model. C57BL/6 female mice (n = 40) were used; half were placed on a moderately iron deficient (ID) diet immediately post-weaning, and the other half were maintained on an iron replete (IR) diet. Half were dosed with H.pylori SS1 at 5 weeks of age, and the remaining mice were sham-dosed. There were 4 study groups: a control group (-Hp, IR diet) as well as 3 experimental groups (-Hp, ID diet; +Hp, IR diet; +Hp,ID diet). All mice were tested in an open field apparatus at 8 weeks postinfection. Independent of dietary iron status, H.pylori -infected mice performed fewer exploratory behaviors in the open field chamber than uninfected mice (p<0.001). Hippocampal gene expression of myelination markers and dopamine receptor 1 was significantly downregulated in mice on an ID diet (both p<0.05), independent of infection status. At 12 months postinfection, hematocrit (Hct) and hemoglobin (Hgb) concentration were significantly lower in +Hp, ID diet mice compared to all other study groups. H.pylori infection caused IDA in mice maintained on a marginal iron diet. The mouse model developed in this study is a useful model to study the neurologic, behavioral, and hematologic impact of the common human co-morbidity of H. pylori infection and IDA. |
| first_indexed | 2024-09-23T09:44:37Z |
| format | Article |
| id | mit-1721.1/109954 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:44:37Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/1099542022-09-30T16:34:53Z Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice Amaya, Aldo Ennis, Kathleen Wang, Timothy C. Georgieff, Michael Burns, Monika Bodi Winn, Caroline M Ge, Zhongming Bakthavatchalu, Vasudevan Fox, James G Broad Institute of MIT and Harvard Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Division of Comparative Medicine Burns, Monika Bodi Winn, Caroline M Ge, Zhongming Bakthavatchalu, Vasudevan Fox, James G Helicobacter pylori (H.pylori), a bacterial pathogen, is a causative agent of gastritis and peptic ulcer disease and is a strong risk factor for development of gastric cancer. Environmental conditions, such as poor dietary iron resulting in iron deficiency anemia (IDA), enhance H.pylori virulence and increases risk for gastric cancer. IDA affects billions of people worldwide, and there is considerable overlap between regions of high IDA and high H.pylori prevalence. The primary aims of our study were to evaluate the effect of H.pylori infection on behavior, iron metabolism, red blood cell indices, and behavioral outcomes following comorbid H. pylori infection and dietary iron deficiency in a mouse model. C57BL/6 female mice (n = 40) were used; half were placed on a moderately iron deficient (ID) diet immediately post-weaning, and the other half were maintained on an iron replete (IR) diet. Half were dosed with H.pylori SS1 at 5 weeks of age, and the remaining mice were sham-dosed. There were 4 study groups: a control group (-Hp, IR diet) as well as 3 experimental groups (-Hp, ID diet; +Hp, IR diet; +Hp,ID diet). All mice were tested in an open field apparatus at 8 weeks postinfection. Independent of dietary iron status, H.pylori -infected mice performed fewer exploratory behaviors in the open field chamber than uninfected mice (p<0.001). Hippocampal gene expression of myelination markers and dopamine receptor 1 was significantly downregulated in mice on an ID diet (both p<0.05), independent of infection status. At 12 months postinfection, hematocrit (Hct) and hemoglobin (Hgb) concentration were significantly lower in +Hp, ID diet mice compared to all other study groups. H.pylori infection caused IDA in mice maintained on a marginal iron diet. The mouse model developed in this study is a useful model to study the neurologic, behavioral, and hematologic impact of the common human co-morbidity of H. pylori infection and IDA. United States. National Institutes of Health (T32OD010978) United States. National Institutes of Health (R01CA093405) United States. National Institutes of Health (P01CA028842-23) National Institute of Environmental Health Sciences (P30ES0022109) 2017-06-16T17:16:47Z 2017-06-16T17:16:47Z 2017-03 2016-10 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/109954 Burns, Monika; Amaya, Aldo; Bodi, Caroline; Ge, Zhongming; Bakthavatchalu, Vasudevan; Ennis, Kathleen; Wang, Timothy C.; Georgieff, Michael and Fox, James G. “Helicobacter Pylori Infection and Low Dietary Iron Alter Behavior, Induce Iron Deficiency Anemia, and Modulate Hippocampal Gene Expression in Female C57BL/6 Mice.” Edited by James R. Connor. PLOS ONE 12, no. 3 (March 2017): e0173108 © 2017 Burns et al https://orcid.org/0000-0002-3418-056X https://orcid.org/0000-0001-9307-6116 en_US http://dx.doi.org/10.1371/journal.pone.0173108 PLoS ONE Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science PLoS |
| spellingShingle | Amaya, Aldo Ennis, Kathleen Wang, Timothy C. Georgieff, Michael Burns, Monika Bodi Winn, Caroline M Ge, Zhongming Bakthavatchalu, Vasudevan Fox, James G Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice |
| title | Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice |
| title_full | Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice |
| title_fullStr | Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice |
| title_full_unstemmed | Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice |
| title_short | Helicobacter pylori infection and low dietary iron alter behavior, induce iron deficiency anemia, and modulate hippocampal gene expression in female C57BL/6 mice |
| title_sort | helicobacter pylori infection and low dietary iron alter behavior induce iron deficiency anemia and modulate hippocampal gene expression in female c57bl 6 mice |
| url | http://hdl.handle.net/1721.1/109954 https://orcid.org/0000-0002-3418-056X https://orcid.org/0000-0001-9307-6116 |
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