Meioc maintains an extended meiotic prophase I in mice
The meiosis-specific chromosomal events of homolog pairing, synapsis, and recombination occur over an extended meiotic prophase I that is many times longer than prophase of mitosis. Here we show that, in mice, maintenance of an extended meiotic prophase I requires the gene Meioc, a germ-cell specifi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2017
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| Online Access: | http://hdl.handle.net/1721.1/110002 https://orcid.org/0000-0002-3387-8608 https://orcid.org/0000-0002-5681-3225 https://orcid.org/0000-0001-9920-3411 |
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| author | Soh, Ying Qi Shirleen Mikedis, Maria M. Kojima, Mina Godfrey, Alexander Kamitsuka Page, David C de Rooij, Dirk G. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Soh, Ying Qi Shirleen Mikedis, Maria M. Kojima, Mina Godfrey, Alexander Kamitsuka Page, David C de Rooij, Dirk G. |
| author_sort | Soh, Ying Qi Shirleen |
| collection | MIT |
| description | The meiosis-specific chromosomal events of homolog pairing, synapsis, and recombination occur over an extended meiotic prophase I that is many times longer than prophase of mitosis. Here we show that, in mice, maintenance of an extended meiotic prophase I requires the gene Meioc, a germ-cell specific factor conserved in most metazoans. In mice, Meioc is expressed in male and female germ cells upon initiation of and throughout meiotic prophase I. Mouse germ cells lacking Meioc initiate meiosis: they undergo pre-meiotic DNA replication, they express proteins involved in synapsis and recombination, and a subset of cells progress as far as the zygotene stage of prophase I. However, cells in early meiotic prophase—as early as the preleptotene stage—proceed to condense their chromosomes and assemble a spindle, as if having progressed to metaphase. Meioc-deficient spermatocytes that have initiated synapsis mis-express CYCLIN A2, which is normally expressed in mitotic spermatogonia, suggesting a failure to properly transition to a meiotic cell cycle program. MEIOC interacts with YTHDC2, and the two proteins pull-down an overlapping set of mitosis-associated transcripts. We conclude that when the meiotic chromosomal program is initiated, Meioc is simultaneously induced so as to extend meiotic prophase. Specifically, MEIOC, together with YTHDC2, promotes a meiotic (as opposed to mitotic) cell cycle program via post-transcriptional control of their target transcripts. |
| first_indexed | 2024-09-23T16:44:32Z |
| format | Article |
| id | mit-1721.1/110002 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:44:32Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/1100022022-09-29T21:12:56Z Meioc maintains an extended meiotic prophase I in mice Soh, Ying Qi Shirleen Mikedis, Maria M. Kojima, Mina Godfrey, Alexander Kamitsuka Page, David C de Rooij, Dirk G. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Soh, Ying Qi Shirleen Mikedis, Maria M. Kojima, Mina Godfrey, Alexander Kamitsuka Page, David C de Rooij, Dirk G. The meiosis-specific chromosomal events of homolog pairing, synapsis, and recombination occur over an extended meiotic prophase I that is many times longer than prophase of mitosis. Here we show that, in mice, maintenance of an extended meiotic prophase I requires the gene Meioc, a germ-cell specific factor conserved in most metazoans. In mice, Meioc is expressed in male and female germ cells upon initiation of and throughout meiotic prophase I. Mouse germ cells lacking Meioc initiate meiosis: they undergo pre-meiotic DNA replication, they express proteins involved in synapsis and recombination, and a subset of cells progress as far as the zygotene stage of prophase I. However, cells in early meiotic prophase—as early as the preleptotene stage—proceed to condense their chromosomes and assemble a spindle, as if having progressed to metaphase. Meioc-deficient spermatocytes that have initiated synapsis mis-express CYCLIN A2, which is normally expressed in mitotic spermatogonia, suggesting a failure to properly transition to a meiotic cell cycle program. MEIOC interacts with YTHDC2, and the two proteins pull-down an overlapping set of mitosis-associated transcripts. We conclude that when the meiotic chromosomal program is initiated, Meioc is simultaneously induced so as to extend meiotic prophase. Specifically, MEIOC, together with YTHDC2, promotes a meiotic (as opposed to mitotic) cell cycle program via post-transcriptional control of their target transcripts. 2017-06-19T13:36:16Z 2017-06-19T13:36:16Z 2017-04 2016-12 Article http://purl.org/eprint/type/JournalArticle 1553-7404 1553-7390 http://hdl.handle.net/1721.1/110002 Soh, Y. Q. Shirleen; Mikedis, Maria M.; Kojima, Mina; Godfrey, Alexander K.; de Rooij, Dirk G. and Page, David C. “Meioc Maintains an Extended Meiotic Prophase I in Mice.” Edited by Paula E. Cohen. PLOS Genetics 13, no. 4 (April 5, 2017): e1006704 © 2017 Soh et al https://orcid.org/0000-0002-3387-8608 https://orcid.org/0000-0002-5681-3225 https://orcid.org/0000-0001-9920-3411 en_US http://dx.doi.org/10.1371/journal.pgen.1006704 PLoS Genetics Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science PLoS |
| spellingShingle | Soh, Ying Qi Shirleen Mikedis, Maria M. Kojima, Mina Godfrey, Alexander Kamitsuka Page, David C de Rooij, Dirk G. Meioc maintains an extended meiotic prophase I in mice |
| title | Meioc maintains an extended meiotic prophase I in mice |
| title_full | Meioc maintains an extended meiotic prophase I in mice |
| title_fullStr | Meioc maintains an extended meiotic prophase I in mice |
| title_full_unstemmed | Meioc maintains an extended meiotic prophase I in mice |
| title_short | Meioc maintains an extended meiotic prophase I in mice |
| title_sort | meioc maintains an extended meiotic prophase i in mice |
| url | http://hdl.handle.net/1721.1/110002 https://orcid.org/0000-0002-3387-8608 https://orcid.org/0000-0002-5681-3225 https://orcid.org/0000-0001-9920-3411 |
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