Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro
Club cells are known to function as regional progenitor cells to repair the bronchiolar epithelium in response to lung damage. By lineage tracing in mice, we have shown recently that club cells also give rise to alveolar type 2 cells (AT2s) and alveolar type 1 cells (AT1s) during the repair of the d...
| Main Authors: | , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2017
|
| Online Access: | http://hdl.handle.net/1721.1/110075 https://orcid.org/0000-0003-1828-0509 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826201139788382208 |
|---|---|
| author | Zheng, Dahai Soh, Boon-Seng Yin, Lu Hu, Guangan Chen, Qingfeng Choi, Hyungwon Han, Jongyoon Chow, Vincent T. K. Chen, Jianzhu |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Zheng, Dahai Soh, Boon-Seng Yin, Lu Hu, Guangan Chen, Qingfeng Choi, Hyungwon Han, Jongyoon Chow, Vincent T. K. Chen, Jianzhu |
| author_sort | Zheng, Dahai |
| collection | MIT |
| description | Club cells are known to function as regional progenitor cells to repair the bronchiolar epithelium in response to lung damage. By lineage tracing in mice, we have shown recently that club cells also give rise to alveolar type 2 cells (AT2s) and alveolar type 1 cells (AT1s) during the repair of the damaged alveolar epithelium. Here, we show that when highly purified, anatomically and phenotypically confirmed club cells are seeded in 3-dimensional culture either in bulk or individually, they proliferate and differentiate into both AT2- and AT1-like cells and form alveolar-like structures. This differentiation was further confirmed by transcriptomic analysis of freshly isolated club cells and their cultured progeny. Freshly isolated club cells express Sca-1 and integrin α6, markers commonly used to characterize lung stem/progenitor cells. Together, current study for the first time isolated highly purified club cells for in vitro study and demonstrated club cells’ capacity to differentiate into alveolar epithelial cells at the single-cell level. |
| first_indexed | 2024-09-23T11:46:50Z |
| format | Article |
| id | mit-1721.1/110075 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:46:50Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1100752022-10-01T05:59:44Z Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro Zheng, Dahai Soh, Boon-Seng Yin, Lu Hu, Guangan Chen, Qingfeng Choi, Hyungwon Han, Jongyoon Chow, Vincent T. K. Chen, Jianzhu Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Koch Institute for Integrative Cancer Research at MIT Hu, Guangan Han, Jongyoon Club cells are known to function as regional progenitor cells to repair the bronchiolar epithelium in response to lung damage. By lineage tracing in mice, we have shown recently that club cells also give rise to alveolar type 2 cells (AT2s) and alveolar type 1 cells (AT1s) during the repair of the damaged alveolar epithelium. Here, we show that when highly purified, anatomically and phenotypically confirmed club cells are seeded in 3-dimensional culture either in bulk or individually, they proliferate and differentiate into both AT2- and AT1-like cells and form alveolar-like structures. This differentiation was further confirmed by transcriptomic analysis of freshly isolated club cells and their cultured progeny. Freshly isolated club cells express Sca-1 and integrin α6, markers commonly used to characterize lung stem/progenitor cells. Together, current study for the first time isolated highly purified club cells for in vitro study and demonstrated club cells’ capacity to differentiate into alveolar epithelial cells at the single-cell level. National Cancer Institute (U.S.) (P30-CA14051) 2017-06-20T18:22:07Z 2017-06-20T18:22:07Z 2017-01 2016-11 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/110075 Zheng, Dahai; Soh, Boon-Seng; Yin, Lu; Hu, Guangan; Chen, Qingfeng; Choi, Hyungwon; Han, Jongyoon; Chow, Vincent T. K. and Chen, Jianzhu. “Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro.” Scientific Reports 7 (January 2017): 41661 © 2017 The Author(s) https://orcid.org/0000-0003-1828-0509 https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1038/srep41661 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Zheng, Dahai Soh, Boon-Seng Yin, Lu Hu, Guangan Chen, Qingfeng Choi, Hyungwon Han, Jongyoon Chow, Vincent T. K. Chen, Jianzhu Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro |
| title | Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro |
| title_full | Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro |
| title_fullStr | Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro |
| title_full_unstemmed | Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro |
| title_short | Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro |
| title_sort | differentiation of club cells to alveolar epithelial cells in vitro |
| url | http://hdl.handle.net/1721.1/110075 https://orcid.org/0000-0003-1828-0509 https://orcid.org/0000-0001-7215-1439 |
| work_keys_str_mv | AT zhengdahai differentiationofclubcellstoalveolarepithelialcellsinvitro AT sohboonseng differentiationofclubcellstoalveolarepithelialcellsinvitro AT yinlu differentiationofclubcellstoalveolarepithelialcellsinvitro AT huguangan differentiationofclubcellstoalveolarepithelialcellsinvitro AT chenqingfeng differentiationofclubcellstoalveolarepithelialcellsinvitro AT choihyungwon differentiationofclubcellstoalveolarepithelialcellsinvitro AT hanjongyoon differentiationofclubcellstoalveolarepithelialcellsinvitro AT chowvincenttk differentiationofclubcellstoalveolarepithelialcellsinvitro AT chenjianzhu differentiationofclubcellstoalveolarepithelialcellsinvitro |