Self-organizing actin patterns shape membrane architecture but not cell mechanics
Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic techniques, we here show evidence of such self-organization of the actin cortex in living HeL...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2017
|
| Online Access: | http://hdl.handle.net/1721.1/110109 https://orcid.org/0000-0002-7019-3907 |
| _version_ | 1826188656923115520 |
|---|---|
| author | Fritzsche, M. Li, D. Colin-York, H. Chang, V. T. Felce, J. H. Sezgin, E. Charras, G. Betzig, E. Eggeling, C. Moeendarbary, Emadaldin |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Fritzsche, M. Li, D. Colin-York, H. Chang, V. T. Felce, J. H. Sezgin, E. Charras, G. Betzig, E. Eggeling, C. Moeendarbary, Emadaldin |
| author_sort | Fritzsche, M. |
| collection | MIT |
| description | Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic techniques, we here show evidence of such self-organization of the actin cortex in living HeLa cells. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. This process is primarily driven by Arp2/3 complex nucleation, but not by myosin motors, which is in contrast to what has been theoretically predicted and observed in vitro. Concomitant measurements of mechanics and plasma membrane fluidity demonstrate that changes in actin patterning alter membrane architecture but occur functionally independent of macroscopic cortex elasticity. Consequently, tuning the activity of the Arp2/3 complex to alter filament assembly may thus be a mechanism allowing cells to adjust their membrane architecture without affecting their macroscopic mechanical properties. |
| first_indexed | 2024-09-23T08:03:01Z |
| format | Article |
| id | mit-1721.1/110109 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:03:01Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1101092022-09-30T07:09:19Z Self-organizing actin patterns shape membrane architecture but not cell mechanics Fritzsche, M. Li, D. Colin-York, H. Chang, V. T. Felce, J. H. Sezgin, E. Charras, G. Betzig, E. Eggeling, C. Moeendarbary, Emadaldin Massachusetts Institute of Technology. Department of Biological Engineering Moeendarbary, Emadaldin Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic techniques, we here show evidence of such self-organization of the actin cortex in living HeLa cells. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. This process is primarily driven by Arp2/3 complex nucleation, but not by myosin motors, which is in contrast to what has been theoretically predicted and observed in vitro. Concomitant measurements of mechanics and plasma membrane fluidity demonstrate that changes in actin patterning alter membrane architecture but occur functionally independent of macroscopic cortex elasticity. Consequently, tuning the activity of the Arp2/3 complex to alter filament assembly may thus be a mechanism allowing cells to adjust their membrane architecture without affecting their macroscopic mechanical properties. Wellcome Trust (WT103883) 2017-06-21T14:28:55Z 2017-06-21T14:28:55Z 2017-02 2016-12 Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/110109 Fritzsche, M.; Li, D.; Colin-York, H.; Chang, V. T.; Moeendarbary, E.; Felce, J. H.; Sezgin, E.; Charras, G.; Betzig, E. and Eggeling, C. “Self-Organizing Actin Patterns Shape Membrane Architecture but Not Cell Mechanics.” Nature Communications 8 (February 2017): 14347 © 2017 The Authors https://orcid.org/0000-0002-7019-3907 en_US http://dx.doi.org/10.1038/ncomms14347 Nature Communications Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Fritzsche, M. Li, D. Colin-York, H. Chang, V. T. Felce, J. H. Sezgin, E. Charras, G. Betzig, E. Eggeling, C. Moeendarbary, Emadaldin Self-organizing actin patterns shape membrane architecture but not cell mechanics |
| title | Self-organizing actin patterns shape membrane architecture but not cell mechanics |
| title_full | Self-organizing actin patterns shape membrane architecture but not cell mechanics |
| title_fullStr | Self-organizing actin patterns shape membrane architecture but not cell mechanics |
| title_full_unstemmed | Self-organizing actin patterns shape membrane architecture but not cell mechanics |
| title_short | Self-organizing actin patterns shape membrane architecture but not cell mechanics |
| title_sort | self organizing actin patterns shape membrane architecture but not cell mechanics |
| url | http://hdl.handle.net/1721.1/110109 https://orcid.org/0000-0002-7019-3907 |
| work_keys_str_mv | AT fritzschem selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT lid selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT colinyorkh selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT changvt selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT felcejh selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT sezgine selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT charrasg selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT betzige selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT eggelingc selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics AT moeendarbaryemadaldin selforganizingactinpatternsshapemembranearchitecturebutnotcellmechanics |