The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization
The kinesin-3 family member KIF1A has been shown to be important for experience dependent neuroplasticity. In Drosophila, amorphic mutations in the KIF1A homolog unc-104 disrupt the formation of mature boutons. Disease associated KIF1A mutations have been associated with motor and sensory dysfunctio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/110113 https://orcid.org/0000-0001-7463-977X |
| _version_ | 1826204605485154304 |
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| author | Hannan, Shabab B. Kern, Jeannine V. Stanchev, Doychin T. Jahn, Thomas R. Rasse, Tobias M. Koc, Baran Zhang, Yao |
| author2 | Picower Institute for Learning and Memory |
| author_facet | Picower Institute for Learning and Memory Hannan, Shabab B. Kern, Jeannine V. Stanchev, Doychin T. Jahn, Thomas R. Rasse, Tobias M. Koc, Baran Zhang, Yao |
| author_sort | Hannan, Shabab B. |
| collection | MIT |
| description | The kinesin-3 family member KIF1A has been shown to be important for experience dependent neuroplasticity. In Drosophila, amorphic mutations in the KIF1A homolog unc-104 disrupt the formation of mature boutons. Disease associated KIF1A mutations have been associated with motor and sensory dysfunctions as well as non-syndromic intellectual disability in humans. A hypomorphic mutation in the forkhead-associated domain of Unc-104, unc-104[superscript bris], impairs active zone maturation resulting in an increased fraction of post-synaptic glutamate receptor fields that lack the active zone scaffolding protein Bruchpilot. Here, we show that the unc-104[superscript bris]mutation causes defects in synaptic transmission as manifested by reduced amplitude of both evoked and miniature excitatory junctional potentials. Structural defects observed in the postsynaptic compartment of mutant NMJs include reduced glutamate receptor field size, and altered glutamate receptor composition. In addition, we observed marked loss of postsynaptic scaffolding proteins and reduced complexity of the sub-synaptic reticulum, which could be rescued by pre- but not postsynaptic expression of unc-104. Our results highlight the importance of kinesin-3 based axonal transport in synaptic transmission and provide novel insights into the role of Unc-104 in synapse maturation. |
| first_indexed | 2024-09-23T12:57:47Z |
| format | Article |
| id | mit-1721.1/110113 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:57:47Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1101132022-10-01T12:12:59Z The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization Hannan, Shabab B. Kern, Jeannine V. Stanchev, Doychin T. Jahn, Thomas R. Rasse, Tobias M. Koc, Baran Zhang, Yao Picower Institute for Learning and Memory Zhang, Yao The kinesin-3 family member KIF1A has been shown to be important for experience dependent neuroplasticity. In Drosophila, amorphic mutations in the KIF1A homolog unc-104 disrupt the formation of mature boutons. Disease associated KIF1A mutations have been associated with motor and sensory dysfunctions as well as non-syndromic intellectual disability in humans. A hypomorphic mutation in the forkhead-associated domain of Unc-104, unc-104[superscript bris], impairs active zone maturation resulting in an increased fraction of post-synaptic glutamate receptor fields that lack the active zone scaffolding protein Bruchpilot. Here, we show that the unc-104[superscript bris]mutation causes defects in synaptic transmission as manifested by reduced amplitude of both evoked and miniature excitatory junctional potentials. Structural defects observed in the postsynaptic compartment of mutant NMJs include reduced glutamate receptor field size, and altered glutamate receptor composition. In addition, we observed marked loss of postsynaptic scaffolding proteins and reduced complexity of the sub-synaptic reticulum, which could be rescued by pre- but not postsynaptic expression of unc-104. Our results highlight the importance of kinesin-3 based axonal transport in synaptic transmission and provide novel insights into the role of Unc-104 in synapse maturation. 2017-06-21T14:53:38Z 2017-06-21T14:53:38Z 2017-03 2015-10 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/110113 Zhang, Yao V. et al. “The KIF1A Homolog Unc-104 Is Important for Spontaneous Release, Postsynaptic Density Maturation and Perisynaptic Scaffold Organization.” Scientific Reports 7 (2017): 38172. https://orcid.org/0000-0001-7463-977X en_US http://dx.doi.org/10.1038/srep38172 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Hannan, Shabab B. Kern, Jeannine V. Stanchev, Doychin T. Jahn, Thomas R. Rasse, Tobias M. Koc, Baran Zhang, Yao The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization |
| title | The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization |
| title_full | The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization |
| title_fullStr | The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization |
| title_full_unstemmed | The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization |
| title_short | The KIF1A homolog Unc-104 is important for spontaneous release, postsynaptic density maturation and perisynaptic scaffold organization |
| title_sort | kif1a homolog unc 104 is important for spontaneous release postsynaptic density maturation and perisynaptic scaffold organization |
| url | http://hdl.handle.net/1721.1/110113 https://orcid.org/0000-0001-7463-977X |
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