Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device
Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | en_US |
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MDPI AG
2017
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| Online Access: | http://hdl.handle.net/1721.1/110164 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826193098856726528 |
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| author | Rowlands, Christopher Park, Jong Kang So, Peter T. C. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Rowlands, Christopher Park, Jong Kang So, Peter T. C. |
| author_sort | Rowlands, Christopher |
| collection | MIT |
| description | Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice. |
| first_indexed | 2024-09-23T09:33:44Z |
| format | Article |
| id | mit-1721.1/110164 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:33:44Z |
| publishDate | 2017 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | mit-1721.1/1101642022-09-30T15:19:41Z Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device Rowlands, Christopher Park, Jong Kang So, Peter T. C. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Laser Biomedical Research Center Park, Jong Kang So, Peter T. C. Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice. United States. National Institutes of Health (5-P41-EB015871-28) United States. National Institutes of Health (2R01EY017656-06) United States. National Institutes of Health (R21-NS091982-01) United States. National Institutes of Health (1-U01-NS090438-01) United States. National Institutes of Health (1U01CA202177-01) United States. National Institutes of Health (1R01HL121386-01A1) 2017-06-22T14:49:18Z 2017-06-22T14:49:18Z 2017-03 2017-02 Article http://purl.org/eprint/type/JournalArticle 2072-666X http://hdl.handle.net/1721.1/110164 Park, Jong; Rowlands, Christopher and So, Peter. “Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device.” Micromachines 8, no. 3 (March 2017): 85 © 2017 The Author(s) https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.3390/mi8030085 Micromachines Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf MDPI AG MDPI |
| spellingShingle | Rowlands, Christopher Park, Jong Kang So, Peter T. C. Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
| title | Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
| title_full | Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
| title_fullStr | Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
| title_full_unstemmed | Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
| title_short | Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
| title_sort | enhanced axial resolution of wide field two photon excitation microscopy by line scanning using a digital micromirror device |
| url | http://hdl.handle.net/1721.1/110164 https://orcid.org/0000-0003-4698-6488 |
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