Engineering a 3D microfluidic culture platform for tumor-treating field application
The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-canc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/110351 https://orcid.org/0000-0002-7232-304X |
| _version_ | 1826204232960704512 |
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| author | Tay, Andy Warkiani, Majid Ebrahimi Yeap, Wei Hseun Wong, Siew Cheng Pavesi, Andrea Adriani, Giulia Kamm, Roger Dale |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Tay, Andy Warkiani, Majid Ebrahimi Yeap, Wei Hseun Wong, Siew Cheng Pavesi, Andrea Adriani, Giulia Kamm, Roger Dale |
| author_sort | Tay, Andy |
| collection | MIT |
| description | The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy. |
| first_indexed | 2024-09-23T12:51:01Z |
| format | Article |
| id | mit-1721.1/110351 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:51:01Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1103512022-09-28T10:27:13Z Engineering a 3D microfluidic culture platform for tumor-treating field application Tay, Andy Warkiani, Majid Ebrahimi Yeap, Wei Hseun Wong, Siew Cheng Pavesi, Andrea Adriani, Giulia Kamm, Roger Dale Massachusetts Institute of Technology. Department of Biological Engineering Singapore-MIT Alliance in Research and Technology (SMART) Pavesi, Andrea Adriani, Giulia Kamm, Roger Dale The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy. 2017-06-28T14:58:32Z 2017-06-28T14:58:32Z 2016-05 2015-12 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/110351 Pavesi, Andrea, Giulia Adriani, Andy Tay, Majid Ebrahimi Warkiani, Wei Hseun Yeap, Siew Cheng Wong, and Roger D. Kamm. “Engineering a 3D Microfluidic Culture Platform for Tumor-Treating Field Application.” Scientific Reports 6, 26584 (May 2016): 1-10 © 2016 Macmillan Publishers Limited, part of Springer Nature. https://orcid.org/0000-0002-7232-304X en_US http://dx.doi.org/10.1038/srep26584 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Scientific Reports |
| spellingShingle | Tay, Andy Warkiani, Majid Ebrahimi Yeap, Wei Hseun Wong, Siew Cheng Pavesi, Andrea Adriani, Giulia Kamm, Roger Dale Engineering a 3D microfluidic culture platform for tumor-treating field application |
| title | Engineering a 3D microfluidic culture platform for tumor-treating field application |
| title_full | Engineering a 3D microfluidic culture platform for tumor-treating field application |
| title_fullStr | Engineering a 3D microfluidic culture platform for tumor-treating field application |
| title_full_unstemmed | Engineering a 3D microfluidic culture platform for tumor-treating field application |
| title_short | Engineering a 3D microfluidic culture platform for tumor-treating field application |
| title_sort | engineering a 3d microfluidic culture platform for tumor treating field application |
| url | http://hdl.handle.net/1721.1/110351 https://orcid.org/0000-0002-7232-304X |
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