A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue
Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exoge...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry, The
2017
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| Online Access: | http://hdl.handle.net/1721.1/110418 https://orcid.org/0000-0002-5910-6948 https://orcid.org/0000-0002-2693-4982 |
| _version_ | 1826204891860697088 |
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| author | Khan, Mustafa Georgiou, John Luyben, Thomas T. Roder, John C. Okamoto, Kenichi Rivera Fuentes, Pablo Marcelo Wrobel, Alexandra T. Zastrow, Melissa L. Lippard, Stephen J. |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Khan, Mustafa Georgiou, John Luyben, Thomas T. Roder, John C. Okamoto, Kenichi Rivera Fuentes, Pablo Marcelo Wrobel, Alexandra T. Zastrow, Melissa L. Lippard, Stephen J. |
| author_sort | Khan, Mustafa |
| collection | MIT |
| description | Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn²⁺ in lysosomes of HeLa cells, endogenous Zn²⁺ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy. |
| first_indexed | 2024-09-23T13:02:42Z |
| format | Article |
| id | mit-1721.1/110418 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:02:42Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/1104182022-10-01T12:46:03Z A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue Khan, Mustafa Georgiou, John Luyben, Thomas T. Roder, John C. Okamoto, Kenichi Rivera Fuentes, Pablo Marcelo Wrobel, Alexandra T. Zastrow, Melissa L. Lippard, Stephen J. Massachusetts Institute of Technology. Department of Chemistry Rivera Fuentes, Pablo Marcelo Wrobel, Alexandra T. Zastrow, Melissa L. Lippard, Stephen J. Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn²⁺ in lysosomes of HeLa cells, endogenous Zn²⁺ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy. National Institute of General Medical Sciences (U.S.) (GM065519) 2017-07-03T14:15:02Z 2017-07-03T14:15:02Z 2015-01 2014-11 Article http://purl.org/eprint/type/JournalArticle 2041-6520 2041-6539 http://hdl.handle.net/1721.1/110418 Rivera-Fuentes, Pablo; Wrobel, Alexandra T.; Zastrow, Melissa L.; Khan, Mustafa; Georgiou, John; Luyben, Thomas T.; Roder, John C.; Okamoto, Kenichi and Lippard, Stephen J. "A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue." Chemical Science 2015, 6 (January 2015): 1944-1948 © 2015 The Royal Society of Chemistry https://orcid.org/0000-0002-5910-6948 https://orcid.org/0000-0002-2693-4982 en_US http://dx.doi.org/10.1039/c4sc03388d Chemical Science Creative Commons Attribution-NonCommercial 3.0 Unported licence http://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry, The Royal Society of Chemistry |
| spellingShingle | Khan, Mustafa Georgiou, John Luyben, Thomas T. Roder, John C. Okamoto, Kenichi Rivera Fuentes, Pablo Marcelo Wrobel, Alexandra T. Zastrow, Melissa L. Lippard, Stephen J. A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| title | A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| title_full | A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| title_fullStr | A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| title_full_unstemmed | A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| title_short | A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| title_sort | far red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue |
| url | http://hdl.handle.net/1721.1/110418 https://orcid.org/0000-0002-5910-6948 https://orcid.org/0000-0002-2693-4982 |
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