Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration
Regeneration requires initiation of programs tailored to the identity of missing parts. Head-versus-tail regeneration in planarians presents a paradigm for study of this phenomenon. After injury, Wnt signaling promotes tail regeneration. We report that wounding elicits expression of the Wnt inhibito...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Association for the Advancement of Science (AAAS)
2017
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| Online Access: | http://hdl.handle.net/1721.1/110540 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1811082746589609984 |
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| author | Petersen, Christian Paul Reddien, Peter |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Petersen, Christian Paul Reddien, Peter |
| author_sort | Petersen, Christian Paul |
| collection | MIT |
| description | Regeneration requires initiation of programs tailored to the identity of missing parts. Head-versus-tail regeneration in planarians presents a paradigm for study of this phenomenon. After injury, Wnt signaling promotes tail regeneration. We report that wounding elicits expression of the Wnt inhibitor notum preferentially at anterior-facing wounds. This expression asymmetry occurs at essentially any wound, even if the anterior pole is intact. Inhibition of notum with RNA interference (RNAi) causes regeneration of an anterior-facing tail instead of a head, and double-RNAi experiments indicate that notum inhibits Wnt signaling to promote head regeneration. notum expression is itself controlled by Wnt signaling, suggesting that regulation of feedback inhibition controls the binary head-tail regeneration outcome. We conclude that local detection of wound orientation with respect to tissue axes results in distinct signaling environments that initiate appropriate regeneration responses. |
| first_indexed | 2024-09-23T12:07:59Z |
| format | Article |
| id | mit-1721.1/110540 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:07:59Z |
| publishDate | 2017 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1105402022-10-01T08:26:15Z Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration Petersen, Christian Paul Reddien, Peter Massachusetts Institute of Technology. Department of Biology Petersen, Christian Paul Reddien, Peter Regeneration requires initiation of programs tailored to the identity of missing parts. Head-versus-tail regeneration in planarians presents a paradigm for study of this phenomenon. After injury, Wnt signaling promotes tail regeneration. We report that wounding elicits expression of the Wnt inhibitor notum preferentially at anterior-facing wounds. This expression asymmetry occurs at essentially any wound, even if the anterior pole is intact. Inhibition of notum with RNA interference (RNAi) causes regeneration of an anterior-facing tail instead of a head, and double-RNAi experiments indicate that notum inhibits Wnt signaling to promote head regeneration. notum expression is itself controlled by Wnt signaling, suggesting that regulation of feedback inhibition controls the binary head-tail regeneration outcome. We conclude that local detection of wound orientation with respect to tissue axes results in distinct signaling environments that initiate appropriate regeneration responses. National Institutes of Health (U.S.) (R01GM080639) American Cancer Society (RSG-07-180-01-DDC) 2017-07-07T17:27:32Z 2017-07-07T17:27:32Z 2011-05 2010-12 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/110540 Petersen, C. P. and Reddien, P. W. "Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration." Science 332, 6031 (May 2011): 852-855 © 2011 American Association for the Advancement of Science https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.1126/science.1202143 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Petersen, Christian Paul Reddien, Peter Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration |
| title | Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration |
| title_full | Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration |
| title_fullStr | Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration |
| title_full_unstemmed | Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration |
| title_short | Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration |
| title_sort | polarized notum activation at wounds inhibits wnt function to promote planarian head regeneration |
| url | http://hdl.handle.net/1721.1/110540 https://orcid.org/0000-0002-5569-333X |
| work_keys_str_mv | AT petersenchristianpaul polarizednotumactivationatwoundsinhibitswntfunctiontopromoteplanarianheadregeneration AT reddienpeter polarizednotumactivationatwoundsinhibitswntfunctiontopromoteplanarianheadregeneration |