Patterning Vascular Networks In Vivo for Tissue Engineering Applications
The ultimate design of functionally therapeutic engineered tissues and organs will rely on our ability to engineer vasculature that can meet tissue-specific metabolic needs. We recently introduced an approach for patterning the formation of functional spatially organized vascular architectures withi...
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2017
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| Online Access: | http://hdl.handle.net/1721.1/110769 https://orcid.org/0000-0002-1293-2097 |
| _version_ | 1811074388417576960 |
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| author | Chaturvedi, Ritika R. Solorzano, Ricardo D. Eyckmans, Jeroen Baranski, Jan D. Stapleton, Sarah Chase Stevens, Kelly R. Bhatia, Sangeeta N Chen, Christopher S. Schwartz, Robert E. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Chaturvedi, Ritika R. Solorzano, Ricardo D. Eyckmans, Jeroen Baranski, Jan D. Stapleton, Sarah Chase Stevens, Kelly R. Bhatia, Sangeeta N Chen, Christopher S. Schwartz, Robert E. |
| author_sort | Chaturvedi, Ritika R. |
| collection | MIT |
| description | The ultimate design of functionally therapeutic engineered tissues and organs will rely on our ability to engineer vasculature that can meet tissue-specific metabolic needs. We recently introduced an approach for patterning the formation of functional spatially organized vascular architectures within engineered tissues in vivo. Here, we now explore the design parameters of this approach and how they impact the vascularization of an engineered tissue construct after implantation. We used micropatterning techniques to organize endothelial cells (ECs) into geometrically defined “cords,” which in turn acted as a template after implantation for the guided formation of patterned capillaries integrated with the host tissue. We demonstrated that the diameter of the cords before implantation impacts the location and density of the resultant capillary network. Inclusion of mural cells to the vascularization response appears primarily to impact the dynamics of vascularization. We established that clinically relevant endothelial sources such as induced pluripotent stem cell-derived ECs and human microvascular endothelial cells can drive vascularization within this system. Finally, we demonstrated the ability to control the juxtaposition of parenchyma with perfused vasculature by implanting cords containing a mixture of both a parenchymal cell type (hepatocytes) and ECs. These findings define important characteristics that will ultimately impact the design of vasculature structures that meet tissue-specific needs. |
| first_indexed | 2024-09-23T09:48:40Z |
| format | Article |
| id | mit-1721.1/110769 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:48:40Z |
| publishDate | 2017 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/1107692024-03-20T02:42:55Z Patterning Vascular Networks In Vivo for Tissue Engineering Applications Chaturvedi, Ritika R. Solorzano, Ricardo D. Eyckmans, Jeroen Baranski, Jan D. Stapleton, Sarah Chase Stevens, Kelly R. Bhatia, Sangeeta N Chen, Christopher S. Schwartz, Robert E. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Stevens, Kelly R. Schwartz, Robert E Bhatia, Sangeeta N Chen, Christopher S. The ultimate design of functionally therapeutic engineered tissues and organs will rely on our ability to engineer vasculature that can meet tissue-specific metabolic needs. We recently introduced an approach for patterning the formation of functional spatially organized vascular architectures within engineered tissues in vivo. Here, we now explore the design parameters of this approach and how they impact the vascularization of an engineered tissue construct after implantation. We used micropatterning techniques to organize endothelial cells (ECs) into geometrically defined “cords,” which in turn acted as a template after implantation for the guided formation of patterned capillaries integrated with the host tissue. We demonstrated that the diameter of the cords before implantation impacts the location and density of the resultant capillary network. Inclusion of mural cells to the vascularization response appears primarily to impact the dynamics of vascularization. We established that clinically relevant endothelial sources such as induced pluripotent stem cell-derived ECs and human microvascular endothelial cells can drive vascularization within this system. Finally, we demonstrated the ability to control the juxtaposition of parenchyma with perfused vasculature by implanting cords containing a mixture of both a parenchymal cell type (hepatocytes) and ECs. These findings define important characteristics that will ultimately impact the design of vasculature structures that meet tissue-specific needs. National Institute of Biomedical Imaging and Bioengineering (U.S.) (Award Number EB000262) National Institute of Biomedical Imaging and Bioengineering (U.S.) (Award Number EB08396) National Institutes of Health (U.S.). National Research Service Awards (1F32DK091007) National Institutes of Health (U.S.). National Research Service Awards (5T32AR007132-35) 2017-07-18T18:05:23Z 2017-07-18T18:05:23Z 2015-02 Article http://purl.org/eprint/type/JournalArticle 1937-3384 1937-3392 http://hdl.handle.net/1721.1/110769 Chaturvedi, Ritika R., Kelly R. Stevens, Ricardo D. Solorzano, Robert E. Schwartz, Jeroen Eyckmans, Jan D. Baranski, Sarah Chase Stapleton, Sangeeta N. Bhatia, and Christopher S. Chen. “Patterning Vascular NetworksIn Vivofor Tissue Engineering Applications.” Tissue Engineering Part C: Methods 21, no. 5 (May 2015): 509–517. https://orcid.org/0000-0002-1293-2097 en_US http://dx.doi.org/10.1089/ten.tec.2014.0258 Tissue Engineering Part C: Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Liebert |
| spellingShingle | Chaturvedi, Ritika R. Solorzano, Ricardo D. Eyckmans, Jeroen Baranski, Jan D. Stapleton, Sarah Chase Stevens, Kelly R. Bhatia, Sangeeta N Chen, Christopher S. Schwartz, Robert E. Patterning Vascular Networks In Vivo for Tissue Engineering Applications |
| title | Patterning Vascular Networks In Vivo for Tissue Engineering Applications |
| title_full | Patterning Vascular Networks In Vivo for Tissue Engineering Applications |
| title_fullStr | Patterning Vascular Networks In Vivo for Tissue Engineering Applications |
| title_full_unstemmed | Patterning Vascular Networks In Vivo for Tissue Engineering Applications |
| title_short | Patterning Vascular Networks In Vivo for Tissue Engineering Applications |
| title_sort | patterning vascular networks in vivo for tissue engineering applications |
| url | http://hdl.handle.net/1721.1/110769 https://orcid.org/0000-0002-1293-2097 |
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