Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage
In this study, the effects of prolonged storage on several biophysical properties of red blood cells (RBCs) were investigated. Single cell deformability was used as an important criterion in determining subgroups of RBCs evolved during storage lesion. A deformability-based microfluidic cell sorting...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
| Published: |
Royal Society of Chemistry, The
2017
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| Online Access: | http://hdl.handle.net/1721.1/110939 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826209367471423488 |
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| author | Huang, Sha Hou, Han Wei Kanias, Tamir Sertorio, Jonas Tadeu Chen, Huichao Sinchar, Derek Gladwin, Mark T. Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Huang, Sha Hou, Han Wei Kanias, Tamir Sertorio, Jonas Tadeu Chen, Huichao Sinchar, Derek Gladwin, Mark T. Han, Jongyoon |
| author_sort | Huang, Sha |
| collection | MIT |
| description | In this study, the effects of prolonged storage on several biophysical properties of red blood cells (RBCs) were investigated. Single cell deformability was used as an important criterion in determining subgroups of RBCs evolved during storage lesion. A deformability-based microfluidic cell sorting technology was applied, which demonstrates the ability to enrich and separate the less deformable subpopulations of stored blood. These less deformable RBC subpopulations were then associated with other important markers such as osmotic fragility indicating cell integrity as well as microparticle content. This work demonstrates a systematic methodology to both monitor and improve banked blood quality, thereby reducing risks related to blood transfusion. |
| first_indexed | 2024-09-23T14:21:24Z |
| format | Article |
| id | mit-1721.1/110939 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:21:24Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/1109392022-10-01T20:49:34Z Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage Huang, Sha Hou, Han Wei Kanias, Tamir Sertorio, Jonas Tadeu Chen, Huichao Sinchar, Derek Gladwin, Mark T. Han, Jongyoon Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Huang, Sha Hou, Han Wei Han, Jongyoon In this study, the effects of prolonged storage on several biophysical properties of red blood cells (RBCs) were investigated. Single cell deformability was used as an important criterion in determining subgroups of RBCs evolved during storage lesion. A deformability-based microfluidic cell sorting technology was applied, which demonstrates the ability to enrich and separate the less deformable subpopulations of stored blood. These less deformable RBC subpopulations were then associated with other important markers such as osmotic fragility indicating cell integrity as well as microparticle content. This work demonstrates a systematic methodology to both monitor and improve banked blood quality, thereby reducing risks related to blood transfusion. United States. Defense Advanced Research Projects Agency (N66001-11-1-4182) 2017-08-14T14:42:03Z 2017-08-14T14:42:03Z 2014-11 2014-07 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/110939 Huang, Sha et al. “Towards Microfluidic-Based Depletion of Stiff and Fragile Human Red Cells That Accumulate During Blood Storage.” Lab Chip 15, 2 (January 2015): 448–458 © 2015 The Royal Society of Chemistry https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1039/c4lc00768a Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry, The PMC |
| spellingShingle | Huang, Sha Hou, Han Wei Kanias, Tamir Sertorio, Jonas Tadeu Chen, Huichao Sinchar, Derek Gladwin, Mark T. Han, Jongyoon Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage |
| title | Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage |
| title_full | Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage |
| title_fullStr | Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage |
| title_full_unstemmed | Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage |
| title_short | Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage |
| title_sort | towards microfluidic based depletion of stiff and fragile human red cells that accumulate during blood storage |
| url | http://hdl.handle.net/1721.1/110939 https://orcid.org/0000-0001-7215-1439 |
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