A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum
Current techniques for cell culture routinely use animal-derived components. Fetal bovine serum (FBS) is the most widely applied supplement, but it often displays significant batch-to-batch variations and is generally not suitable for clinical applications in humans. A robust and xeno-free alternati...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Biomed Central Ltd.
2017
|
| Online Access: | http://hdl.handle.net/1721.1/111008 |
| _version_ | 1826215557648613376 |
|---|---|
| author | Castells-Sala, C. Martorell, J. Balcells-Camps, Mercedes |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Castells-Sala, C. Martorell, J. Balcells-Camps, Mercedes |
| author_sort | Castells-Sala, C. |
| collection | MIT |
| description | Current techniques for cell culture routinely use animal-derived components. Fetal bovine serum (FBS) is the most widely applied supplement, but it often displays significant batch-to-batch variations and is generally not suitable for clinical applications in humans. A robust and xeno-free alternative to FBS is of high interest for cellular therapies, from early in vitro testing to clinical trials in human subjects. In the current work, a highly consistent human plasma derived supplement (SCC) has been tested, as a potential substitute of FBS in primary human vascular cells culture. Our results show that SCC is able to support proliferation, preserve cellular morphology and potentiate functionality analogously to FBS. We conclude that SCC is a viable substitute of FBS for culture and expansion of cells in advanced therapies using human vascular cells and fibroblasts. |
| first_indexed | 2024-09-23T16:35:05Z |
| format | Article |
| id | mit-1721.1/111008 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:35:05Z |
| publishDate | 2017 |
| publisher | Biomed Central Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/1110082022-10-02T08:19:46Z A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum Castells-Sala, C. Martorell, J. Balcells-Camps, Mercedes Massachusetts Institute of Technology. Institute for Medical Engineering & Science Balcells-Camps, Mercedes Current techniques for cell culture routinely use animal-derived components. Fetal bovine serum (FBS) is the most widely applied supplement, but it often displays significant batch-to-batch variations and is generally not suitable for clinical applications in humans. A robust and xeno-free alternative to FBS is of high interest for cellular therapies, from early in vitro testing to clinical trials in human subjects. In the current work, a highly consistent human plasma derived supplement (SCC) has been tested, as a potential substitute of FBS in primary human vascular cells culture. Our results show that SCC is able to support proliferation, preserve cellular morphology and potentiate functionality analogously to FBS. We conclude that SCC is a viable substitute of FBS for culture and expansion of cells in advanced therapies using human vascular cells and fibroblasts. 2017-08-23T19:52:45Z 2017-08-23T19:52:45Z 2017-08 2017-03 2017-08-20T03:49:13Z Article http://purl.org/eprint/type/JournalArticle 2045-3701 http://hdl.handle.net/1721.1/111008 Castells-Sala, C. et al. "A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum." Cell & Bioscience 7 (August 2017): 41 © 2017 The Author(s) en http://dx.doi.org/10.1186/s13578-017-0164-4 Cell & Bioscience Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ The Author(s) application/pdf Biomed Central Ltd. BioMed Central |
| spellingShingle | Castells-Sala, C. Martorell, J. Balcells-Camps, Mercedes A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| title | A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| title_full | A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| title_fullStr | A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| title_full_unstemmed | A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| title_short | A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| title_sort | human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum |
| url | http://hdl.handle.net/1721.1/111008 |
| work_keys_str_mv | AT castellssalac ahumanplasmaderivedsupplementpreservesfunctionofhumanvascularcellsinabsenceoffetalbovineserum AT martorellj ahumanplasmaderivedsupplementpreservesfunctionofhumanvascularcellsinabsenceoffetalbovineserum AT balcellscampsmercedes ahumanplasmaderivedsupplementpreservesfunctionofhumanvascularcellsinabsenceoffetalbovineserum AT castellssalac humanplasmaderivedsupplementpreservesfunctionofhumanvascularcellsinabsenceoffetalbovineserum AT martorellj humanplasmaderivedsupplementpreservesfunctionofhumanvascularcellsinabsenceoffetalbovineserum AT balcellscampsmercedes humanplasmaderivedsupplementpreservesfunctionofhumanvascularcellsinabsenceoffetalbovineserum |