Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment
Tissue engineering approaches using growth factor-functionalized acellular scaffolds to support and guide repair driven by endogenous cells are thought to require a careful balance between cell recruitment and growth factor release kinetics. The objective of this study was to identify a growth facto...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc
2017
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| Online Access: | http://hdl.handle.net/1721.1/111147 https://orcid.org/0000-0002-4121-8183 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1826196304726851584 |
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| author | Pancoast, James R. Mroszczyk, Keri A. Young, Whitney T. Lee, Richard T. Frisbie, David D. Kisiday, John D. Liebesny, Paul Hancock Byun, Sangwon Hung, Han-Hwa K Grodzinsky, Alan J |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Pancoast, James R. Mroszczyk, Keri A. Young, Whitney T. Lee, Richard T. Frisbie, David D. Kisiday, John D. Liebesny, Paul Hancock Byun, Sangwon Hung, Han-Hwa K Grodzinsky, Alan J |
| author_sort | Pancoast, James R. |
| collection | MIT |
| description | Tissue engineering approaches using growth factor-functionalized acellular scaffolds to support and guide repair driven by endogenous cells are thought to require a careful balance between cell recruitment and growth factor release kinetics. The objective of this study was to identify a growth factor combination that accelerates progenitor cell migration into self-assembling peptide hydrogels in the context of cartilage defect repair. A novel 3D gel-to-gel migration assay enabled quantification of the chemotactic impact of platelet-derived growth factor-BB (PDGF-BB), heparin-binding insulin-like growth factor-1 (HB-IGF-1), and transforming growth factor-β1 (TGF-β1) on progenitor cells derived from subchondral bovine trabecular bone (bone-marrow progenitor cells, BM-PCs) encapsulated in the peptide hydrogel [KLDL]3. Only the combination of PDGF-BB and TGF-β1 stimulated significant migration of BM-PCs over a 4-day period, measured by confocal microscopy. Both PDGF-BB and TGF-β1 were slowly released from the gel, as measured using their 125I-labeled forms, and they remained significantly present in the gel at 4 days. In the context of augmenting microfracture surgery for cartilage repair, our strategy of delivering chemotactic and proanabolic growth factors in KLD may provide the necessary local stimulus to help increase defect cellularity, providing more cells to generate repair tissue. |
| first_indexed | 2024-09-23T10:24:47Z |
| format | Article |
| id | mit-1721.1/111147 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:24:47Z |
| publishDate | 2017 |
| publisher | Mary Ann Liebert, Inc |
| record_format | dspace |
| spelling | mit-1721.1/1111472022-09-26T17:43:32Z Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment Pancoast, James R. Mroszczyk, Keri A. Young, Whitney T. Lee, Richard T. Frisbie, David D. Kisiday, John D. Liebesny, Paul Hancock Byun, Sangwon Hung, Han-Hwa K Grodzinsky, Alan J Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Liebesny, Paul Hancock Byun, Sangwon Hung, Han-Hwa K Grodzinsky, Alan J Tissue engineering approaches using growth factor-functionalized acellular scaffolds to support and guide repair driven by endogenous cells are thought to require a careful balance between cell recruitment and growth factor release kinetics. The objective of this study was to identify a growth factor combination that accelerates progenitor cell migration into self-assembling peptide hydrogels in the context of cartilage defect repair. A novel 3D gel-to-gel migration assay enabled quantification of the chemotactic impact of platelet-derived growth factor-BB (PDGF-BB), heparin-binding insulin-like growth factor-1 (HB-IGF-1), and transforming growth factor-β1 (TGF-β1) on progenitor cells derived from subchondral bovine trabecular bone (bone-marrow progenitor cells, BM-PCs) encapsulated in the peptide hydrogel [KLDL]3. Only the combination of PDGF-BB and TGF-β1 stimulated significant migration of BM-PCs over a 4-day period, measured by confocal microscopy. Both PDGF-BB and TGF-β1 were slowly released from the gel, as measured using their 125I-labeled forms, and they remained significantly present in the gel at 4 days. In the context of augmenting microfracture surgery for cartilage repair, our strategy of delivering chemotactic and proanabolic growth factors in KLD may provide the necessary local stimulus to help increase defect cellularity, providing more cells to generate repair tissue. National Institutes of Health (U.S.) (Grant AR060331) 2017-09-07T15:54:36Z 2017-09-07T15:54:36Z 2016-06 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/111147 Liebesny, Paul H. et al. “Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment.” Tissue Engineering Part A 22, 13–14 (July 2016): 917–927 © 2016 Mary Ann Liebert, Inc https://orcid.org/0000-0002-4121-8183 https://orcid.org/0000-0002-4942-3456 en_US http://dx.doi.org/10.1089/ten.TEA.2015.0524 Tissue Engineering Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc Mary Ann Liebert |
| spellingShingle | Pancoast, James R. Mroszczyk, Keri A. Young, Whitney T. Lee, Richard T. Frisbie, David D. Kisiday, John D. Liebesny, Paul Hancock Byun, Sangwon Hung, Han-Hwa K Grodzinsky, Alan J Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment |
| title | Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment |
| title_full | Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment |
| title_fullStr | Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment |
| title_full_unstemmed | Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment |
| title_short | Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment |
| title_sort | growth factor mediated migration of bone marrow progenitor cells for accelerated scaffold recruitment |
| url | http://hdl.handle.net/1721.1/111147 https://orcid.org/0000-0002-4121-8183 https://orcid.org/0000-0002-4942-3456 |
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