Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc
2017
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| Online Access: | http://hdl.handle.net/1721.1/111152 https://orcid.org/0000-0002-4890-424X |
| _version_ | 1811075555679797248 |
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| author | Carter, Bradley S. Cortés-Campos, Christian McCammon, Jasmine M. Chen, Xiao Sive, Hazel L. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Carter, Bradley S. Cortés-Campos, Christian McCammon, Jasmine M. Chen, Xiao Sive, Hazel L. |
| author_sort | Carter, Bradley S. |
| collection | MIT |
| description | Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented. |
| first_indexed | 2024-09-23T10:08:07Z |
| format | Article |
| id | mit-1721.1/111152 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:08:07Z |
| publishDate | 2017 |
| publisher | Mary Ann Liebert, Inc |
| record_format | dspace |
| spelling | mit-1721.1/1111522022-09-30T19:06:52Z Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays Carter, Bradley S. Cortés-Campos, Christian McCammon, Jasmine M. Chen, Xiao Sive, Hazel L. Massachusetts Institute of Technology. Department of Biology Chen, Xiao Sive, Hazel L Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented. National Science Foundation (U.S.) (IOS-1258087) 2017-09-07T17:34:30Z 2017-09-07T17:34:30Z 2016-08 Article http://purl.org/eprint/type/JournalArticle 1545-8547 1557-8542 http://hdl.handle.net/1721.1/111152 Carter, Bradley S. et al. “Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays.” Zebrafish 14, 1 (February 2017): 73–76 © 2017 Mary Ann Liebert, Inc https://orcid.org/0000-0002-4890-424X en_US http://dx.doi.org/10.1089/zeb.2016.1326 Zebrafish Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc Mary Ann Liebert |
| spellingShingle | Carter, Bradley S. Cortés-Campos, Christian McCammon, Jasmine M. Chen, Xiao Sive, Hazel L. Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays |
| title | Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays |
| title_full | Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays |
| title_fullStr | Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays |
| title_full_unstemmed | Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays |
| title_short | Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays |
| title_sort | validation of protein knockout in mutant zebrafish lines using in vitro translation assays |
| url | http://hdl.handle.net/1721.1/111152 https://orcid.org/0000-0002-4890-424X |
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