Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays

Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however...

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Bibliographic Details
Main Authors: Carter, Bradley S., Cortés-Campos, Christian, McCammon, Jasmine M., Chen, Xiao, Sive, Hazel L.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Mary Ann Liebert, Inc 2017
Online Access:http://hdl.handle.net/1721.1/111152
https://orcid.org/0000-0002-4890-424X

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