Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however...
Main Authors: | Carter, Bradley S., Cortés-Campos, Christian, McCammon, Jasmine M., Chen, Xiao, Sive, Hazel L. |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | en_US |
Published: |
Mary Ann Liebert, Inc
2017
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Online Access: | http://hdl.handle.net/1721.1/111152 https://orcid.org/0000-0002-4890-424X |
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