Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows
The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated...
| Principais autores: | , , , , , , , , , |
|---|---|
| Outros Autores: | |
| Formato: | Artigo |
| Idioma: | en_US |
| Publicado em: |
Elsevier
2017
|
| Acesso em linha: | http://hdl.handle.net/1721.1/111177 https://orcid.org/0000-0003-1019-4390 https://orcid.org/0000-0002-1348-9584 |
| _version_ | 1826205172414545920 |
|---|---|
| author | Lopes, J.C. de Matos, L.F. Harper, M.T. Giallongo, F. Oh, J. Kindermann, M. Duval, S. Hristov, A.N. Gruen, Danielle Sarah Ono, Shuhei |
| author2 | Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences Lopes, J.C. de Matos, L.F. Harper, M.T. Giallongo, F. Oh, J. Kindermann, M. Duval, S. Hristov, A.N. Gruen, Danielle Sarah Ono, Shuhei |
| author_sort | Lopes, J.C. |
| collection | MIT |
| description | The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatments: control and 3NOP (60 mg/kg of feed dry matter). Compared with the control, 3NOP decreased methane emission by 31% and increased hydrogen emission from undetectable to 1.33 g/d. Methane emissions per kilogram of dry matter intake and milk yield were also decreased 34% by 3NOP. Milk production and composition were not affected by 3NOP, except milk fat concentration was increased compared with the control. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment, but acetate concentration tended to be lower and acetate-to-propionate ratio was lower for 3NOP compared with the control. The 3NOP decreased the molar proportion of acetate and increase those of propionate, butyrate, valerate, and isovalerate. Deuterium-to-hydrogen ratios of methane and the abundance of ¹³CH₃D were similar between treatments. Compared with the control, minor (4‰) depletion in the ¹³C/¹²C ratio was observed for 3NOP. Genus composition of methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) was not affected by 3NOP, but the proportion of methanogens in the total cell counts tended to be decreased by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents in this experiment, was also not affected by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased and Butyrivibrio spp. was increased by 3NOP. This experiment demonstrated that a substantial inhibition of enteric methane emission by 3NOP in dairy cows was accompanied with increased hydrogen emission and decreased acetate-to-propionate ratio; however, neither an effect on rumen archaeal community composition nor a significant change in the isotope composition of methane was observed. |
| first_indexed | 2024-09-23T13:08:52Z |
| format | Article |
| id | mit-1721.1/111177 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:08:52Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1111772022-09-28T12:14:34Z Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows Lopes, J.C. de Matos, L.F. Harper, M.T. Giallongo, F. Oh, J. Kindermann, M. Duval, S. Hristov, A.N. Gruen, Danielle Sarah Ono, Shuhei Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences Ono, Shuhei Gruen, Danielle Sarah Ono, Shuhei The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatments: control and 3NOP (60 mg/kg of feed dry matter). Compared with the control, 3NOP decreased methane emission by 31% and increased hydrogen emission from undetectable to 1.33 g/d. Methane emissions per kilogram of dry matter intake and milk yield were also decreased 34% by 3NOP. Milk production and composition were not affected by 3NOP, except milk fat concentration was increased compared with the control. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment, but acetate concentration tended to be lower and acetate-to-propionate ratio was lower for 3NOP compared with the control. The 3NOP decreased the molar proportion of acetate and increase those of propionate, butyrate, valerate, and isovalerate. Deuterium-to-hydrogen ratios of methane and the abundance of ¹³CH₃D were similar between treatments. Compared with the control, minor (4‰) depletion in the ¹³C/¹²C ratio was observed for 3NOP. Genus composition of methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) was not affected by 3NOP, but the proportion of methanogens in the total cell counts tended to be decreased by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents in this experiment, was also not affected by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased and Butyrivibrio spp. was increased by 3NOP. This experiment demonstrated that a substantial inhibition of enteric methane emission by 3NOP in dairy cows was accompanied with increased hydrogen emission and decreased acetate-to-propionate ratio; however, neither an effect on rumen archaeal community composition nor a significant change in the isotope composition of methane was observed. 2017-09-12T15:06:35Z 2017-09-12T15:06:35Z 2016-04 2015-12 Article http://purl.org/eprint/type/JournalArticle 0022-0302 1525-3198 http://hdl.handle.net/1721.1/111177 Lopes, J.C. et al. “Effect of 3-Nitrooxypropanol on Methane and Hydrogen Emissions, Methane Isotopic Signature, and Ruminal Fermentation in Dairy Cows.” Journal of Dairy Science 99, 7 (July 2016): 5335–5344 © 2016 Elsevier https://orcid.org/0000-0003-1019-4390 https://orcid.org/0000-0002-1348-9584 en_US https://doi.org/10.3168/jds.2015-10832 Journal of Dairy Science Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Prof. Ono via Michael Noga |
| spellingShingle | Lopes, J.C. de Matos, L.F. Harper, M.T. Giallongo, F. Oh, J. Kindermann, M. Duval, S. Hristov, A.N. Gruen, Danielle Sarah Ono, Shuhei Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
| title | Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
| title_full | Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
| title_fullStr | Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
| title_full_unstemmed | Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
| title_short | Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
| title_sort | effect of 3 nitrooxypropanol on methane and hydrogen emissions methane isotopic signature and ruminal fermentation in dairy cows |
| url | http://hdl.handle.net/1721.1/111177 https://orcid.org/0000-0003-1019-4390 https://orcid.org/0000-0002-1348-9584 |
| work_keys_str_mv | AT lopesjc effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT dematoslf effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT harpermt effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT giallongof effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT ohj effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT kindermannm effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT duvals effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT hristovan effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT gruendaniellesarah effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows AT onoshuhei effectof3nitrooxypropanolonmethaneandhydrogenemissionsmethaneisotopicsignatureandruminalfermentationindairycows |