Development and Applications of CRISPR-Cas9 for Genome Engineering
Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex g...
| Main Authors: | , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/1721.1/111575 https://orcid.org/0000-0003-2782-2509 |
| _version_ | 1811089696722255872 |
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| author | Hsu, Patrick Lander, Eric Steven Zhang, Feng |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hsu, Patrick Lander, Eric Steven Zhang, Feng |
| author_sort | Hsu, Patrick |
| collection | MIT |
| description | Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Cas9-mediated genetic perturbation is simple and scalable, empowering researchers to elucidate the functional organization of the genome at the systems level and establish causal linkages between genetic variations and biological phenotypes. In this Review, we describe the development and applications of Cas9 for a variety of research or translational applications while highlighting challenges as well as future directions. Derived from a remarkable microbial defense system, Cas9 is driving innovative applications from basic biology to biotechnology and medicine. |
| first_indexed | 2024-09-23T14:22:57Z |
| format | Article |
| id | mit-1721.1/111575 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:22:57Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1115752022-10-01T21:00:28Z Development and Applications of CRISPR-Cas9 for Genome Engineering Hsu, Patrick Lander, Eric Steven Zhang, Feng Massachusetts Institute of Technology. Department of Biological Engineering McGovern Institute for Brain Research at MIT Hsu, Patrick Lander, Eric Steven Zhang, Feng Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Cas9-mediated genetic perturbation is simple and scalable, empowering researchers to elucidate the functional organization of the genome at the systems level and establish causal linkages between genetic variations and biological phenotypes. In this Review, we describe the development and applications of Cas9 for a variety of research or translational applications while highlighting challenges as well as future directions. Derived from a remarkable microbial defense system, Cas9 is driving innovative applications from basic biology to biotechnology and medicine. 2017-09-15T20:04:07Z 2017-09-15T20:04:07Z 2014-06 Article http://purl.org/eprint/type/JournalArticle 0092-8674 1097-4172 http://hdl.handle.net/1721.1/111575 Hsu, Patrick D. et al. "Development and Applications of CRISPR-Cas9 for Genome Engineering." Cell 157, 6 (June 2014): 1262-1278 © 2014 Elsevier Inc https://orcid.org/0000-0003-2782-2509 en_US http://dx.doi.org/10.1016/j.cell.2014.05.010 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Hsu, Patrick Lander, Eric Steven Zhang, Feng Development and Applications of CRISPR-Cas9 for Genome Engineering |
| title | Development and Applications of CRISPR-Cas9 for Genome Engineering |
| title_full | Development and Applications of CRISPR-Cas9 for Genome Engineering |
| title_fullStr | Development and Applications of CRISPR-Cas9 for Genome Engineering |
| title_full_unstemmed | Development and Applications of CRISPR-Cas9 for Genome Engineering |
| title_short | Development and Applications of CRISPR-Cas9 for Genome Engineering |
| title_sort | development and applications of crispr cas9 for genome engineering |
| url | http://hdl.handle.net/1721.1/111575 https://orcid.org/0000-0003-2782-2509 |
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